Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20000 and BPHYT_RS20005 are separated by 353 nucleotides BPHYT_RS20005 and BPHYT_RS20010 are separated by 10 nucleotides
BPHYT_RS20000: BPHYT_RS20000 - serine/threonine protein kinase, at 29,770 to 31,245
_RS20000
BPHYT_RS20005: BPHYT_RS20005 - chlorogenate esterase, at 31,599 to 33,332
_RS20005
BPHYT_RS20010: BPHYT_RS20010 - membrane protein, at 33,343 to 34,482
_RS20010
Position (kb) 31
32
33
34 Strain fitness (log2 ratio) -2
-1
0
1 at 31.052 kb on + strand, within BPHYT_RS20000 at 31.052 kb on + strand, within BPHYT_RS20000 at 31.142 kb on + strand at 31.299 kb on + strand at 31.382 kb on + strand at 31.382 kb on + strand at 31.383 kb on - strand at 31.537 kb on - strand at 31.537 kb on - strand at 31.919 kb on - strand, within BPHYT_RS20005 at 31.969 kb on + strand, within BPHYT_RS20005 at 31.970 kb on - strand, within BPHYT_RS20005 at 32.263 kb on + strand, within BPHYT_RS20005 at 32.263 kb on + strand, within BPHYT_RS20005 at 32.264 kb on - strand, within BPHYT_RS20005 at 32.639 kb on - strand, within BPHYT_RS20005 at 32.963 kb on - strand, within BPHYT_RS20005 at 33.458 kb on + strand, within BPHYT_RS20010 at 33.501 kb on - strand, within BPHYT_RS20010 at 33.560 kb on + strand, within BPHYT_RS20010 at 33.561 kb on - strand, within BPHYT_RS20010 at 33.998 kb on + strand, within BPHYT_RS20010 at 34.046 kb on + strand, within BPHYT_RS20010 at 34.070 kb on + strand, within BPHYT_RS20010 at 34.112 kb on + strand, within BPHYT_RS20010
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 31,052 + BPHYT_RS20000 0.87 -1.2 31,052 + BPHYT_RS20000 0.87 -0.2 31,142 + -2.2 31,299 + -1.2 31,382 + +0.5 31,382 + +0.6 31,383 - +0.7 31,537 - -1.4 31,537 - -0.3 31,919 - BPHYT_RS20005 0.18 +0.5 31,969 + BPHYT_RS20005 0.21 +0.6 31,970 - BPHYT_RS20005 0.21 -1.1 32,263 + BPHYT_RS20005 0.38 -0.4 32,263 + BPHYT_RS20005 0.38 -0.7 32,264 - BPHYT_RS20005 0.38 +0.2 32,639 - BPHYT_RS20005 0.60 +0.3 32,963 - BPHYT_RS20005 0.79 +0.4 33,458 + BPHYT_RS20010 0.10 -0.2 33,501 - BPHYT_RS20010 0.14 +0.7 33,560 + BPHYT_RS20010 0.19 -0.0 33,561 - BPHYT_RS20010 0.19 +0.9 33,998 + BPHYT_RS20010 0.57 +0.0 34,046 + BPHYT_RS20010 0.62 -1.2 34,070 + BPHYT_RS20010 0.64 +0.5 34,112 + BPHYT_RS20010 0.67 -0.2
Or see this region's nucleotide sequence