Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS19505 and BPHYT_RS19510 are separated by 315 nucleotides BPHYT_RS19510 and BPHYT_RS19515 are separated by 53 nucleotides BPHYT_RS19515 and BPHYT_RS19520 are separated by 147 nucleotides
BPHYT_RS19505: BPHYT_RS19505 - LysR family transcriptional regulator, at 4,390,216 to 4,391,115
_RS19505
BPHYT_RS19510: BPHYT_RS19510 - adenylate cyclase, at 4,391,431 to 4,391,967
_RS19510
BPHYT_RS19515: BPHYT_RS19515 - AsnC family transcriptional regulator, at 4,392,021 to 4,392,512
_RS19515
BPHYT_RS19520: BPHYT_RS19520 - phenylalanine 4-monooxygenase, at 4,392,660 to 4,393,550
_RS19520
Position (kb)
4391
4392 Strain fitness (log2 ratio)
-1
0
1
2 at 4390.481 kb on + strand, within BPHYT_RS19505 at 4390.481 kb on + strand, within BPHYT_RS19505 at 4390.482 kb on - strand, within BPHYT_RS19505 at 4390.648 kb on + strand, within BPHYT_RS19505 at 4390.651 kb on + strand, within BPHYT_RS19505 at 4390.651 kb on + strand, within BPHYT_RS19505 at 4390.651 kb on + strand, within BPHYT_RS19505 at 4390.652 kb on - strand, within BPHYT_RS19505 at 4390.652 kb on - strand, within BPHYT_RS19505 at 4390.652 kb on - strand, within BPHYT_RS19505 at 4390.833 kb on + strand, within BPHYT_RS19505 at 4391.037 kb on + strand at 4391.038 kb on - strand at 4391.171 kb on + strand at 4391.172 kb on - strand at 4391.653 kb on + strand, within BPHYT_RS19510 at 4391.654 kb on - strand, within BPHYT_RS19510 at 4391.654 kb on - strand, within BPHYT_RS19510 at 4391.749 kb on + strand, within BPHYT_RS19510 at 4391.749 kb on + strand, within BPHYT_RS19510 at 4391.749 kb on + strand, within BPHYT_RS19510 at 4391.749 kb on + strand, within BPHYT_RS19510 at 4391.749 kb on + strand, within BPHYT_RS19510 at 4391.750 kb on - strand, within BPHYT_RS19510 at 4391.750 kb on - strand, within BPHYT_RS19510 at 4391.750 kb on - strand, within BPHYT_RS19510 at 4391.795 kb on + strand, within BPHYT_RS19510 at 4391.795 kb on + strand, within BPHYT_RS19510 at 4391.965 kb on + strand at 4391.965 kb on + strand at 4392.105 kb on + strand, within BPHYT_RS19515 at 4392.106 kb on - strand, within BPHYT_RS19515 at 4392.193 kb on + strand, within BPHYT_RS19515 at 4392.194 kb on - strand, within BPHYT_RS19515 at 4392.436 kb on - strand, within BPHYT_RS19515 at 4392.436 kb on - strand, within BPHYT_RS19515 at 4392.610 kb on + strand at 4392.753 kb on + strand, within BPHYT_RS19520 at 4392.789 kb on + strand, within BPHYT_RS19520
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 4,390,481 + BPHYT_RS19505 0.29 +1.5 4,390,481 + BPHYT_RS19505 0.29 +0.2 4,390,482 - BPHYT_RS19505 0.30 -0.0 4,390,648 + BPHYT_RS19505 0.48 +0.4 4,390,651 + BPHYT_RS19505 0.48 -0.3 4,390,651 + BPHYT_RS19505 0.48 +1.4 4,390,651 + BPHYT_RS19505 0.48 -0.7 4,390,652 - BPHYT_RS19505 0.48 -0.7 4,390,652 - BPHYT_RS19505 0.48 -1.3 4,390,652 - BPHYT_RS19505 0.48 +0.6 4,390,833 + BPHYT_RS19505 0.69 +0.1 4,391,037 + -0.3 4,391,038 - +1.1 4,391,171 + +0.6 4,391,172 - +0.3 4,391,653 + BPHYT_RS19510 0.41 +0.8 4,391,654 - BPHYT_RS19510 0.42 -0.0 4,391,654 - BPHYT_RS19510 0.42 -0.7 4,391,749 + BPHYT_RS19510 0.59 -0.5 4,391,749 + BPHYT_RS19510 0.59 +0.1 4,391,749 + BPHYT_RS19510 0.59 -0.2 4,391,749 + BPHYT_RS19510 0.59 -0.1 4,391,749 + BPHYT_RS19510 0.59 -0.5 4,391,750 - BPHYT_RS19510 0.59 +0.4 4,391,750 - BPHYT_RS19510 0.59 -0.0 4,391,750 - BPHYT_RS19510 0.59 -0.3 4,391,795 + BPHYT_RS19510 0.68 -0.1 4,391,795 + BPHYT_RS19510 0.68 +0.3 4,391,965 + -0.5 4,391,965 + +0.5 4,392,105 + BPHYT_RS19515 0.17 +2.3 4,392,106 - BPHYT_RS19515 0.17 -0.4 4,392,193 + BPHYT_RS19515 0.35 -1.2 4,392,194 - BPHYT_RS19515 0.35 +0.9 4,392,436 - BPHYT_RS19515 0.84 -1.2 4,392,436 - BPHYT_RS19515 0.84 +0.3 4,392,610 + +1.4 4,392,753 + BPHYT_RS19520 0.10 +1.9 4,392,789 + BPHYT_RS19520 0.14 -0.4
Or see this region's nucleotide sequence