Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS17610 and BPHYT_RS17615 are separated by 70 nucleotides BPHYT_RS17615 and BPHYT_RS17620 are separated by 95 nucleotides BPHYT_RS17620 and BPHYT_RS17625 are separated by 21 nucleotides
BPHYT_RS17610: BPHYT_RS17610 - peptidase, at 3,985,435 to 3,985,944
_RS17610
BPHYT_RS17615: BPHYT_RS17615 - Glutathione S-transferase domain, at 3,986,015 to 3,986,626
_RS17615
BPHYT_RS17620: BPHYT_RS17620 - cytochrome C, at 3,986,722 to 3,987,477
_RS17620
BPHYT_RS17625: BPHYT_RS17625 - cytochrome B, at 3,987,499 to 3,988,878
_RS17625
Position (kb)
3986
3987
3988 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3985.901 kb on + strand at 3985.901 kb on + strand at 3985.902 kb on - strand at 3985.919 kb on + strand at 3986.724 kb on - strand at 3986.724 kb on - strand at 3986.731 kb on - strand at 3986.888 kb on - strand, within BPHYT_RS17620 at 3987.066 kb on + strand, within BPHYT_RS17620 at 3987.066 kb on + strand, within BPHYT_RS17620 at 3987.093 kb on - strand, within BPHYT_RS17620 at 3987.093 kb on - strand, within BPHYT_RS17620 at 3987.098 kb on + strand, within BPHYT_RS17620 at 3987.099 kb on - strand, within BPHYT_RS17620 at 3987.099 kb on - strand, within BPHYT_RS17620 at 3987.282 kb on - strand, within BPHYT_RS17620 at 3987.282 kb on - strand, within BPHYT_RS17620 at 3987.318 kb on - strand, within BPHYT_RS17620 at 3987.323 kb on - strand, within BPHYT_RS17620 at 3987.323 kb on - strand, within BPHYT_RS17620 at 3987.463 kb on - strand at 3987.463 kb on - strand at 3987.501 kb on - strand at 3987.588 kb on - strand at 3987.588 kb on - strand at 3987.588 kb on - strand at 3987.640 kb on - strand, within BPHYT_RS17625 at 3987.913 kb on + strand, within BPHYT_RS17625 at 3987.914 kb on - strand, within BPHYT_RS17625 at 3987.914 kb on - strand, within BPHYT_RS17625 at 3987.965 kb on + strand, within BPHYT_RS17625 at 3987.965 kb on + strand, within BPHYT_RS17625 at 3987.966 kb on - strand, within BPHYT_RS17625 at 3988.052 kb on + strand, within BPHYT_RS17625 at 3988.053 kb on - strand, within BPHYT_RS17625 at 3988.136 kb on + strand, within BPHYT_RS17625 at 3988.137 kb on - strand, within BPHYT_RS17625 at 3988.139 kb on + strand, within BPHYT_RS17625 at 3988.139 kb on + strand, within BPHYT_RS17625 at 3988.140 kb on - strand, within BPHYT_RS17625 at 3988.334 kb on + strand, within BPHYT_RS17625 at 3988.335 kb on - strand, within BPHYT_RS17625
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 3,985,901 + -2.2 3,985,901 + -1.3 3,985,902 - +1.4 3,985,919 + +1.2 3,986,724 - +0.1 3,986,724 - -0.1 3,986,731 - -2.0 3,986,888 - BPHYT_RS17620 0.22 -1.8 3,987,066 + BPHYT_RS17620 0.46 -2.3 3,987,066 + BPHYT_RS17620 0.46 -1.6 3,987,093 - BPHYT_RS17620 0.49 -2.0 3,987,093 - BPHYT_RS17620 0.49 -1.6 3,987,098 + BPHYT_RS17620 0.50 +2.2 3,987,099 - BPHYT_RS17620 0.50 -0.3 3,987,099 - BPHYT_RS17620 0.50 -1.2 3,987,282 - BPHYT_RS17620 0.74 -1.1 3,987,282 - BPHYT_RS17620 0.74 -1.0 3,987,318 - BPHYT_RS17620 0.79 -3.5 3,987,323 - BPHYT_RS17620 0.79 -2.1 3,987,323 - BPHYT_RS17620 0.79 -1.1 3,987,463 - -0.4 3,987,463 - -1.2 3,987,501 - +1.2 3,987,588 - +0.6 3,987,588 - -1.2 3,987,588 - -0.9 3,987,640 - BPHYT_RS17625 0.10 -1.7 3,987,913 + BPHYT_RS17625 0.30 -2.0 3,987,914 - BPHYT_RS17625 0.30 -0.0 3,987,914 - BPHYT_RS17625 0.30 -1.4 3,987,965 + BPHYT_RS17625 0.34 -1.2 3,987,965 + BPHYT_RS17625 0.34 -0.6 3,987,966 - BPHYT_RS17625 0.34 -0.8 3,988,052 + BPHYT_RS17625 0.40 -0.9 3,988,053 - BPHYT_RS17625 0.40 -1.8 3,988,136 + BPHYT_RS17625 0.46 +0.5 3,988,137 - BPHYT_RS17625 0.46 -1.6 3,988,139 + BPHYT_RS17625 0.46 -0.3 3,988,139 + BPHYT_RS17625 0.46 -2.5 3,988,140 - BPHYT_RS17625 0.46 -1.4 3,988,334 + BPHYT_RS17625 0.61 -1.2 3,988,335 - BPHYT_RS17625 0.61 -1.6
Or see this region's nucleotide sequence