Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS16345 and BPHYT_RS16350 overlap by 4 nucleotides BPHYT_RS16350 and BPHYT_RS16355 are separated by 149 nucleotides BPHYT_RS16355 and BPHYT_RS16360 are separated by 7 nucleotides
BPHYT_RS16345: BPHYT_RS16345 - succinyl-CoA ligase [ADP-forming] subunit beta, at 3,709,039 to 3,710,208
_RS16345
BPHYT_RS16350: BPHYT_RS16350 - hypothetical protein, at 3,710,205 to 3,710,945
_RS16350
BPHYT_RS16355: BPHYT_RS16355 - recombination protein RecX, at 3,711,095 to 3,711,802
_RS16355
BPHYT_RS16360: BPHYT_RS16360 - recombinase RecA, at 3,711,810 to 3,712,886
_RS16360
Position (kb)
3710
3711 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3709.454 kb on + strand, within BPHYT_RS16345 at 3709.455 kb on - strand, within BPHYT_RS16345 at 3709.455 kb on - strand, within BPHYT_RS16345 at 3709.655 kb on + strand, within BPHYT_RS16345 at 3709.656 kb on - strand, within BPHYT_RS16345 at 3709.883 kb on + strand, within BPHYT_RS16345 at 3709.884 kb on - strand, within BPHYT_RS16345 at 3709.884 kb on - strand, within BPHYT_RS16345 at 3709.884 kb on - strand, within BPHYT_RS16345 at 3710.192 kb on + strand at 3710.193 kb on - strand at 3710.339 kb on + strand, within BPHYT_RS16350 at 3710.340 kb on - strand, within BPHYT_RS16350 at 3710.340 kb on - strand, within BPHYT_RS16350 at 3710.356 kb on + strand, within BPHYT_RS16350 at 3710.357 kb on - strand, within BPHYT_RS16350 at 3710.435 kb on + strand, within BPHYT_RS16350 at 3710.436 kb on - strand, within BPHYT_RS16350 at 3710.666 kb on - strand at 3710.881 kb on + strand at 3710.882 kb on - strand at 3710.882 kb on - strand at 3710.882 kb on - strand at 3710.882 kb on - strand at 3710.989 kb on + strand at 3710.989 kb on + strand at 3710.989 kb on + strand at 3710.990 kb on - strand at 3710.990 kb on - strand at 3710.996 kb on + strand at 3711.054 kb on + strand at 3711.054 kb on + strand at 3711.054 kb on + strand at 3711.054 kb on + strand at 3711.054 kb on + strand at 3711.055 kb on - strand at 3711.055 kb on - strand at 3711.055 kb on - strand at 3711.097 kb on - strand at 3711.320 kb on + strand, within BPHYT_RS16355 at 3711.321 kb on - strand, within BPHYT_RS16355 at 3711.495 kb on + strand, within BPHYT_RS16355 at 3711.510 kb on - strand, within BPHYT_RS16355 at 3711.534 kb on - strand, within BPHYT_RS16355 at 3711.616 kb on - strand, within BPHYT_RS16355 at 3711.797 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 3,709,454 + BPHYT_RS16345 0.35 +3.7 3,709,455 - BPHYT_RS16345 0.36 +1.4 3,709,455 - BPHYT_RS16345 0.36 +0.6 3,709,655 + BPHYT_RS16345 0.53 +0.8 3,709,656 - BPHYT_RS16345 0.53 +1.6 3,709,883 + BPHYT_RS16345 0.72 +1.8 3,709,884 - BPHYT_RS16345 0.72 +1.2 3,709,884 - BPHYT_RS16345 0.72 +1.0 3,709,884 - BPHYT_RS16345 0.72 +1.7 3,710,192 + +0.5 3,710,193 - +1.2 3,710,339 + BPHYT_RS16350 0.18 +2.0 3,710,340 - BPHYT_RS16350 0.18 -1.4 3,710,340 - BPHYT_RS16350 0.18 -1.2 3,710,356 + BPHYT_RS16350 0.20 +1.9 3,710,357 - BPHYT_RS16350 0.21 +0.5 3,710,435 + BPHYT_RS16350 0.31 +1.7 3,710,436 - BPHYT_RS16350 0.31 +0.3 3,710,666 - +1.2 3,710,881 + -0.3 3,710,882 - +0.9 3,710,882 - -0.7 3,710,882 - +0.3 3,710,882 - +0.9 3,710,989 + -0.2 3,710,989 + -0.5 3,710,989 + -0.4 3,710,990 - -0.0 3,710,990 - +1.8 3,710,996 + +0.3 3,711,054 + -0.2 3,711,054 + -1.9 3,711,054 + -0.8 3,711,054 + -0.1 3,711,054 + +0.1 3,711,055 - -1.2 3,711,055 - +1.4 3,711,055 - -0.1 3,711,097 - -2.1 3,711,320 + BPHYT_RS16355 0.32 -1.1 3,711,321 - BPHYT_RS16355 0.32 -0.8 3,711,495 + BPHYT_RS16355 0.56 -0.3 3,711,510 - BPHYT_RS16355 0.59 -0.9 3,711,534 - BPHYT_RS16355 0.62 -2.1 3,711,616 - BPHYT_RS16355 0.74 -1.2 3,711,797 + -0.1
Or see this region's nucleotide sequence