Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14395 and BPHYT_RS14400 are separated by 4 nucleotides BPHYT_RS14400 and BPHYT_RS14405 are separated by 83 nucleotides BPHYT_RS14405 and BPHYT_RS14410 are separated by 101 nucleotides BPHYT_RS14410 and BPHYT_RS14415 are separated by 117 nucleotides
BPHYT_RS14395: BPHYT_RS14395 - sugar dehydratase, at 3,285,787 to 3,286,830
_RS14395
BPHYT_RS14400: BPHYT_RS14400 - anti-sigma factor, at 3,286,835 to 3,287,464
_RS14400
BPHYT_RS14405: BPHYT_RS14405 - RNA polymerase sigma factor RpoE, at 3,287,548 to 3,288,147
_RS14405
BPHYT_RS14410: BPHYT_RS14410 - hypothetical protein, at 3,288,249 to 3,288,632
_RS14410
BPHYT_RS14415: BPHYT_RS14415 - 3-oxoacyl-ACP synthase, at 3,288,750 to 3,289,988
_RS14415
Position (kb)
3287
3288
3289 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3286.746 kb on + strand at 3286.801 kb on - strand at 3286.836 kb on + strand at 3286.836 kb on + strand at 3286.930 kb on - strand, within BPHYT_RS14400 at 3287.314 kb on - strand, within BPHYT_RS14400 at 3287.314 kb on - strand, within BPHYT_RS14400 at 3287.357 kb on - strand, within BPHYT_RS14400 at 3287.479 kb on + strand at 3287.479 kb on + strand at 3287.480 kb on - strand at 3287.480 kb on - strand at 3287.485 kb on + strand at 3287.485 kb on + strand at 3287.486 kb on - strand at 3287.514 kb on - strand at 3288.193 kb on - strand at 3288.193 kb on - strand at 3288.218 kb on - strand at 3288.218 kb on - strand at 3288.324 kb on + strand, within BPHYT_RS14410 at 3288.494 kb on - strand, within BPHYT_RS14410 at 3288.546 kb on - strand, within BPHYT_RS14410 at 3288.583 kb on + strand, within BPHYT_RS14410
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 3,286,746 + +0.7 3,286,801 - +1.7 3,286,836 + +2.1 3,286,836 + +2.1 3,286,930 - BPHYT_RS14400 0.15 -0.7 3,287,314 - BPHYT_RS14400 0.76 +2.3 3,287,314 - BPHYT_RS14400 0.76 +2.9 3,287,357 - BPHYT_RS14400 0.83 +2.4 3,287,479 + +1.4 3,287,479 + +2.0 3,287,480 - -0.3 3,287,480 - +1.3 3,287,485 + +2.1 3,287,485 + +1.4 3,287,486 - -0.2 3,287,514 - +1.1 3,288,193 - +1.0 3,288,193 - +0.7 3,288,218 - +0.7 3,288,218 - +0.6 3,288,324 + BPHYT_RS14410 0.20 +2.2 3,288,494 - BPHYT_RS14410 0.64 -0.3 3,288,546 - BPHYT_RS14410 0.77 +1.1 3,288,583 + BPHYT_RS14410 0.87 -0.1
Or see this region's nucleotide sequence