Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS13625 and BPHYT_RS13630 are separated by 158 nucleotides BPHYT_RS13630 and BPHYT_RS13635 are separated by 29 nucleotides BPHYT_RS13635 and BPHYT_RS13640 are separated by 246 nucleotides BPHYT_RS13640 and BPHYT_RS13645 are separated by 103 nucleotides
BPHYT_RS13625: BPHYT_RS13625 - phenylalanine--tRNA ligase alpha subunit, at 3,106,841 to 3,107,854
_RS13625
BPHYT_RS13630: BPHYT_RS13630 - 50S ribosomal protein L20, at 3,108,013 to 3,108,372
_RS13630
BPHYT_RS13635: BPHYT_RS13635 - 50S ribosomal protein L35, at 3,108,402 to 3,108,599
_RS13635
BPHYT_RS13640: BPHYT_RS13640 - translation initiation factor IF-3, at 3,108,846 to 3,109,316
_RS13640
BPHYT_RS13645: BPHYT_RS13645 - threonine--tRNA ligase, at 3,109,420 to 3,111,327
_RS13645
Position (kb)
3108
3109
3110 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3108.735 kb on - strand at 3108.735 kb on - strand at 3108.735 kb on - strand at 3109.557 kb on - strand at 3109.591 kb on - strand at 3109.592 kb on + strand at 3109.593 kb on - strand at 3109.593 kb on - strand at 3109.593 kb on - strand at 3109.597 kb on - strand at 3109.597 kb on - strand at 3109.597 kb on - strand at 3109.597 kb on - strand at 3109.597 kb on - strand at 3109.597 kb on - strand at 3109.597 kb on - strand at 3109.625 kb on - strand, within BPHYT_RS13645 at 3109.671 kb on - strand, within BPHYT_RS13645 at 3109.872 kb on + strand, within BPHYT_RS13645 at 3109.928 kb on + strand, within BPHYT_RS13645 at 3109.929 kb on - strand, within BPHYT_RS13645 at 3109.929 kb on - strand, within BPHYT_RS13645 at 3109.929 kb on - strand, within BPHYT_RS13645 at 3109.946 kb on + strand, within BPHYT_RS13645 at 3110.108 kb on + strand, within BPHYT_RS13645 at 3110.108 kb on + strand, within BPHYT_RS13645 at 3110.108 kb on + strand, within BPHYT_RS13645 at 3110.109 kb on - strand, within BPHYT_RS13645 at 3110.109 kb on - strand, within BPHYT_RS13645 at 3110.109 kb on - strand, within BPHYT_RS13645 at 3110.128 kb on - strand, within BPHYT_RS13645 at 3110.128 kb on - strand, within BPHYT_RS13645 at 3110.176 kb on - strand, within BPHYT_RS13645 at 3110.176 kb on - strand, within BPHYT_RS13645 at 3110.242 kb on - strand, within BPHYT_RS13645 at 3110.268 kb on - strand, within BPHYT_RS13645 at 3110.268 kb on - strand, within BPHYT_RS13645
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 3,108,735 - -1.1 3,108,735 - -0.6 3,108,735 - +0.6 3,109,557 - +3.2 3,109,591 - +3.5 3,109,592 + +2.8 3,109,593 - +2.0 3,109,593 - +2.4 3,109,593 - +2.6 3,109,597 - +3.0 3,109,597 - +3.1 3,109,597 - +2.5 3,109,597 - +3.0 3,109,597 - +2.8 3,109,597 - +3.3 3,109,597 - +3.1 3,109,625 - BPHYT_RS13645 0.11 +1.2 3,109,671 - BPHYT_RS13645 0.13 +2.2 3,109,872 + BPHYT_RS13645 0.24 +2.6 3,109,928 + BPHYT_RS13645 0.27 +1.8 3,109,929 - BPHYT_RS13645 0.27 +1.9 3,109,929 - BPHYT_RS13645 0.27 +1.6 3,109,929 - BPHYT_RS13645 0.27 +1.7 3,109,946 + BPHYT_RS13645 0.28 +3.2 3,110,108 + BPHYT_RS13645 0.36 +2.4 3,110,108 + BPHYT_RS13645 0.36 +2.5 3,110,108 + BPHYT_RS13645 0.36 +1.9 3,110,109 - BPHYT_RS13645 0.36 +1.4 3,110,109 - BPHYT_RS13645 0.36 +2.4 3,110,109 - BPHYT_RS13645 0.36 +1.8 3,110,128 - BPHYT_RS13645 0.37 +2.2 3,110,128 - BPHYT_RS13645 0.37 +1.5 3,110,176 - BPHYT_RS13645 0.40 +2.6 3,110,176 - BPHYT_RS13645 0.40 +2.2 3,110,242 - BPHYT_RS13645 0.43 +2.3 3,110,268 - BPHYT_RS13645 0.44 +2.0 3,110,268 - BPHYT_RS13645 0.44 +1.7
Or see this region's nucleotide sequence