Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS11975

Experiment: 36hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS11965 and BPHYT_RS11970 are separated by 69 nucleotidesBPHYT_RS11970 and BPHYT_RS11975 are separated by 179 nucleotidesBPHYT_RS11975 and BPHYT_RS11980 are separated by 701 nucleotides BPHYT_RS11965: BPHYT_RS11965 - alkylhydroperoxidase, at 2,723,595 to 2,724,074 _RS11965 BPHYT_RS11970: BPHYT_RS11970 - cupin, at 2,724,144 to 2,724,551 _RS11970 BPHYT_RS11975: BPHYT_RS11975 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase, at 2,724,731 to 2,725,939 _RS11975 BPHYT_RS11980: BPHYT_RS11980 - LysR family transcriptional regulator, at 2,726,641 to 2,727,678 _RS11980 Position (kb) 2724 2725 2726Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 2723.916 kb on + strand, within BPHYT_RS11965at 2724.482 kb on + strand, within BPHYT_RS11970at 2724.719 kb on + strandat 2725.292 kb on - strand, within BPHYT_RS11975at 2725.752 kb on - strand, within BPHYT_RS11975at 2725.786 kb on + strand, within BPHYT_RS11975at 2725.791 kb on - strand, within BPHYT_RS11975at 2726.501 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1
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2,723,916 + BPHYT_RS11965 0.67 +0.8
2,724,482 + BPHYT_RS11970 0.83 -1.8
2,724,719 + +3.8
2,725,292 - BPHYT_RS11975 0.46 +0.6
2,725,752 - BPHYT_RS11975 0.84 -0.8
2,725,786 + BPHYT_RS11975 0.87 -1.0
2,725,791 - BPHYT_RS11975 0.88 +0.4
2,726,501 + +0.2

Or see this region's nucleotide sequence