Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS10915 and BPHYT_RS10920 are separated by 331 nucleotides BPHYT_RS10920 and BPHYT_RS10925 are separated by 321 nucleotides BPHYT_RS10925 and BPHYT_RS10930 are separated by 86 nucleotides BPHYT_RS10930 and BPHYT_RS10935 are separated by 67 nucleotides
BPHYT_RS10915: BPHYT_RS10915 - radical SAM protein, at 2,484,196 to 2,485,617
_RS10915
BPHYT_RS10920: BPHYT_RS10920 - cold-shock protein, at 2,485,949 to 2,486,152
_RS10920
BPHYT_RS10925: BPHYT_RS10925 - aldehyde dehydrogenase, at 2,486,474 to 2,487,919
_RS10925
BPHYT_RS10930: BPHYT_RS10930 - GCN5 family N-acetyltransferase, at 2,488,006 to 2,488,605
_RS10930
BPHYT_RS10935: BPHYT_RS10935 - 2-keto-3-deoxygluconate permease, at 2,488,673 to 2,489,698
_RS10935
Position (kb)
2486
2487
2488 Strain fitness (log2 ratio)
-1
0
1 at 2485.678 kb on + strand at 2485.679 kb on - strand at 2485.711 kb on + strand at 2487.716 kb on + strand, within BPHYT_RS10925 at 2487.717 kb on - strand, within BPHYT_RS10925 at 2487.881 kb on + strand at 2487.917 kb on + strand at 2488.175 kb on - strand, within BPHYT_RS10930 at 2488.353 kb on + strand, within BPHYT_RS10930 at 2488.366 kb on + strand, within BPHYT_RS10930 at 2488.542 kb on + strand, within BPHYT_RS10930 at 2488.543 kb on - strand, within BPHYT_RS10930 at 2488.635 kb on + strand at 2488.639 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 2,485,678 + +0.5 2,485,679 - -1.5 2,485,711 + +0.6 2,487,716 + BPHYT_RS10925 0.86 -0.3 2,487,717 - BPHYT_RS10925 0.86 -0.8 2,487,881 + +0.1 2,487,917 + +0.6 2,488,175 - BPHYT_RS10930 0.28 +1.0 2,488,353 + BPHYT_RS10930 0.58 -1.1 2,488,366 + BPHYT_RS10930 0.60 +0.7 2,488,542 + BPHYT_RS10930 0.89 +0.4 2,488,543 - BPHYT_RS10930 0.90 -0.9 2,488,635 + -0.5 2,488,639 + -0.4
Or see this region's nucleotide sequence