Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09340 and BPHYT_RS09345 are separated by 95 nucleotides BPHYT_RS09345 and BPHYT_RS09350 are separated by 85 nucleotides BPHYT_RS09350 and BPHYT_RS09355 are separated by 130 nucleotides
BPHYT_RS09340: BPHYT_RS09340 - hypothetical protein, at 2,117,652 to 2,118,299
_RS09340
BPHYT_RS09345: BPHYT_RS09345 - TetR family transcriptional regulator, at 2,118,395 to 2,119,009
_RS09345
BPHYT_RS09350: BPHYT_RS09350 - hydrolase, at 2,119,095 to 2,120,237
_RS09350
BPHYT_RS09355: BPHYT_RS09355 - LysR family transcriptional regulator, at 2,120,368 to 2,121,276
_RS09355
Position (kb)
2119
2120
2121 Strain fitness (log2 ratio)
-2
-1
0
1 at 2118.243 kb on + strand at 2118.335 kb on - strand at 2118.365 kb on + strand at 2118.365 kb on + strand at 2118.365 kb on + strand at 2118.366 kb on - strand at 2118.366 kb on - strand at 2118.366 kb on - strand at 2118.402 kb on + strand at 2118.402 kb on + strand at 2118.403 kb on - strand at 2118.403 kb on - strand at 2118.859 kb on - strand, within BPHYT_RS09345 at 2118.866 kb on - strand, within BPHYT_RS09345 at 2119.035 kb on - strand at 2119.079 kb on - strand at 2119.090 kb on - strand at 2119.387 kb on + strand, within BPHYT_RS09350 at 2119.388 kb on - strand, within BPHYT_RS09350 at 2119.388 kb on - strand, within BPHYT_RS09350 at 2119.540 kb on - strand, within BPHYT_RS09350 at 2119.824 kb on + strand, within BPHYT_RS09350 at 2119.896 kb on + strand, within BPHYT_RS09350 at 2119.897 kb on - strand, within BPHYT_RS09350 at 2119.915 kb on - strand, within BPHYT_RS09350 at 2119.915 kb on - strand, within BPHYT_RS09350 at 2120.772 kb on - strand, within BPHYT_RS09355 at 2120.772 kb on - strand, within BPHYT_RS09355 at 2120.801 kb on - strand, within BPHYT_RS09355
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 2,118,243 + +1.3 2,118,335 - -0.9 2,118,365 + -2.0 2,118,365 + +0.3 2,118,365 + +0.5 2,118,366 - +1.3 2,118,366 - +0.1 2,118,366 - -0.4 2,118,402 + +0.9 2,118,402 + +0.3 2,118,403 - -0.2 2,118,403 - +0.9 2,118,859 - BPHYT_RS09345 0.75 +0.3 2,118,866 - BPHYT_RS09345 0.77 +0.6 2,119,035 - +0.8 2,119,079 - +0.1 2,119,090 - +1.3 2,119,387 + BPHYT_RS09350 0.26 -0.1 2,119,388 - BPHYT_RS09350 0.26 -0.2 2,119,388 - BPHYT_RS09350 0.26 -1.0 2,119,540 - BPHYT_RS09350 0.39 -0.4 2,119,824 + BPHYT_RS09350 0.64 -2.2 2,119,896 + BPHYT_RS09350 0.70 -0.3 2,119,897 - BPHYT_RS09350 0.70 -0.6 2,119,915 - BPHYT_RS09350 0.72 +0.1 2,119,915 - BPHYT_RS09350 0.72 +0.5 2,120,772 - BPHYT_RS09355 0.44 +0.8 2,120,772 - BPHYT_RS09355 0.44 -0.0 2,120,801 - BPHYT_RS09355 0.48 +1.1
Or see this region's nucleotide sequence