Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS07250 and BPHYT_RS07255 overlap by 4 nucleotides BPHYT_RS07255 and BPHYT_RS07260 overlap by 4 nucleotides BPHYT_RS07260 and BPHYT_RS07265 are separated by 30 nucleotides BPHYT_RS07265 and BPHYT_RS07270 are separated by 14 nucleotides
BPHYT_RS07250: BPHYT_RS07250 - 4-hydroxy-2-oxovalerate aldolase, at 1,646,430 to 1,647,473
_RS07250
BPHYT_RS07255: BPHYT_RS07255 - 4-oxalocrotonate decarboxylase, at 1,647,470 to 1,648,243
_RS07255
BPHYT_RS07260: BPHYT_RS07260 - 2-aminomuconate deaminase, at 1,648,240 to 1,648,671
_RS07260
BPHYT_RS07265: BPHYT_RS07265 - 3-hydroxyanthranilate 3,4-dioxygenase, at 1,648,702 to 1,649,226
_RS07265
BPHYT_RS07270: BPHYT_RS07270 - aminocarboxymuconate-semialdehyde decarboxylase, at 1,649,241 to 1,650,263
_RS07270
Position (kb)
1647
1648
1649 Strain fitness (log2 ratio)
-1
0
1
2 at 1646.565 kb on - strand, within BPHYT_RS07250 at 1646.858 kb on + strand, within BPHYT_RS07250 at 1646.926 kb on - strand, within BPHYT_RS07250 at 1647.339 kb on + strand, within BPHYT_RS07250 at 1647.650 kb on - strand, within BPHYT_RS07255 at 1647.669 kb on + strand, within BPHYT_RS07255 at 1647.669 kb on + strand, within BPHYT_RS07255 at 1647.670 kb on - strand, within BPHYT_RS07255 at 1648.230 kb on - strand at 1648.669 kb on + strand at 1649.016 kb on - strand, within BPHYT_RS07265
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 1,646,565 - BPHYT_RS07250 0.13 +0.3 1,646,858 + BPHYT_RS07250 0.41 -0.1 1,646,926 - BPHYT_RS07250 0.48 -0.1 1,647,339 + BPHYT_RS07250 0.87 +0.3 1,647,650 - BPHYT_RS07255 0.23 -0.8 1,647,669 + BPHYT_RS07255 0.26 +0.7 1,647,669 + BPHYT_RS07255 0.26 -1.5 1,647,670 - BPHYT_RS07255 0.26 +2.4 1,648,230 - -0.5 1,648,669 + -0.7 1,649,016 - BPHYT_RS07265 0.60 +0.2
Or see this region's nucleotide sequence