Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS06195 and BPHYT_RS06200 are separated by 5 nucleotides BPHYT_RS06200 and BPHYT_RS06205 are separated by 15 nucleotides BPHYT_RS06205 and BPHYT_RS06210 are separated by 85 nucleotides
BPHYT_RS06195: BPHYT_RS06195 - membrane protein, at 1,414,038 to 1,415,384
_RS06195
BPHYT_RS06200: BPHYT_RS06200 - glycosyl transferase, at 1,415,390 to 1,416,649
_RS06200
BPHYT_RS06205: BPHYT_RS06205 - hypothetical protein, at 1,416,665 to 1,416,985
_RS06205
BPHYT_RS06210: BPHYT_RS06210 - hypothetical protein, at 1,417,071 to 1,417,619
_RS06210
Position (kb) 1415
1416
1417 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 1414.833 kb on + strand, within BPHYT_RS06195 at 1415.052 kb on - strand, within BPHYT_RS06195 at 1415.264 kb on - strand at 1415.452 kb on + strand at 1415.614 kb on - strand at 1415.636 kb on + strand, within BPHYT_RS06200 at 1415.636 kb on + strand, within BPHYT_RS06200 at 1415.636 kb on + strand, within BPHYT_RS06200 at 1415.636 kb on + strand, within BPHYT_RS06200 at 1415.636 kb on + strand, within BPHYT_RS06200 at 1415.637 kb on - strand, within BPHYT_RS06200 at 1415.688 kb on + strand, within BPHYT_RS06200 at 1415.740 kb on + strand, within BPHYT_RS06200 at 1415.883 kb on - strand, within BPHYT_RS06200 at 1415.915 kb on + strand, within BPHYT_RS06200 at 1416.140 kb on + strand, within BPHYT_RS06200 at 1416.290 kb on + strand, within BPHYT_RS06200 at 1416.291 kb on - strand, within BPHYT_RS06200 at 1416.750 kb on + strand, within BPHYT_RS06205 at 1416.751 kb on - strand, within BPHYT_RS06205 at 1416.751 kb on - strand, within BPHYT_RS06205 at 1416.858 kb on + strand, within BPHYT_RS06205 at 1416.858 kb on + strand, within BPHYT_RS06205 at 1416.858 kb on + strand, within BPHYT_RS06205 at 1416.859 kb on - strand, within BPHYT_RS06205 at 1416.859 kb on - strand, within BPHYT_RS06205 at 1416.859 kb on - strand, within BPHYT_RS06205 at 1416.864 kb on + strand, within BPHYT_RS06205 at 1417.065 kb on - strand at 1417.159 kb on + strand, within BPHYT_RS06210 at 1417.465 kb on - strand, within BPHYT_RS06210 at 1417.584 kb on - strand at 1417.584 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 1,414,833 + BPHYT_RS06195 0.59 +1.0 1,415,052 - BPHYT_RS06195 0.75 +2.2 1,415,264 - +2.2 1,415,452 + -1.6 1,415,614 - +0.2 1,415,636 + BPHYT_RS06200 0.20 -3.1 1,415,636 + BPHYT_RS06200 0.20 -2.3 1,415,636 + BPHYT_RS06200 0.20 -4.3 1,415,636 + BPHYT_RS06200 0.20 -1.2 1,415,636 + BPHYT_RS06200 0.20 -3.5 1,415,637 - BPHYT_RS06200 0.20 -0.5 1,415,688 + BPHYT_RS06200 0.24 -1.6 1,415,740 + BPHYT_RS06200 0.28 -0.9 1,415,883 - BPHYT_RS06200 0.39 +2.2 1,415,915 + BPHYT_RS06200 0.42 -3.2 1,416,140 + BPHYT_RS06200 0.60 +0.2 1,416,290 + BPHYT_RS06200 0.71 -2.3 1,416,291 - BPHYT_RS06200 0.72 -1.6 1,416,750 + BPHYT_RS06205 0.26 -0.1 1,416,751 - BPHYT_RS06205 0.27 +0.4 1,416,751 - BPHYT_RS06205 0.27 -1.5 1,416,858 + BPHYT_RS06205 0.60 -1.3 1,416,858 + BPHYT_RS06205 0.60 +1.4 1,416,858 + BPHYT_RS06205 0.60 +0.2 1,416,859 - BPHYT_RS06205 0.60 +0.4 1,416,859 - BPHYT_RS06205 0.60 -0.8 1,416,859 - BPHYT_RS06205 0.60 +0.3 1,416,864 + BPHYT_RS06205 0.62 -0.4 1,417,065 - -2.4 1,417,159 + BPHYT_RS06210 0.16 +0.5 1,417,465 - BPHYT_RS06210 0.72 +2.2 1,417,584 - -0.6 1,417,584 - -0.2
Or see this region's nucleotide sequence