Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS05390

Experiment: 36hr PHYTO3 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS05385 and BPHYT_RS05390 are separated by 825 nucleotidesBPHYT_RS05390 and BPHYT_RS05395 are separated by 39 nucleotidesBPHYT_RS05395 and BPHYT_RS05400 are separated by 344 nucleotides BPHYT_RS05385: BPHYT_RS05385 - short-chain dehydrogenase, at 1,238,177 to 1,238,926 _RS05385 BPHYT_RS05390: BPHYT_RS05390 - isochorismatase, at 1,239,752 to 1,240,399 _RS05390 BPHYT_RS05395: BPHYT_RS05395 - hypothetical protein, at 1,240,439 to 1,240,627 _RS05395 BPHYT_RS05400: BPHYT_RS05400 - hypothetical protein, at 1,240,972 to 1,241,418 _RS05400 Position (kb) 1239 1240 1241Strain fitness (log2 ratio) -1 0 1 2at 1238.818 kb on - strand, within BPHYT_RS05385at 1238.818 kb on - strand, within BPHYT_RS05385at 1238.902 kb on - strandat 1239.100 kb on + strandat 1239.101 kb on - strandat 1239.160 kb on + strandat 1239.213 kb on - strandat 1239.771 kb on - strandat 1240.065 kb on + strand, within BPHYT_RS05390at 1240.249 kb on + strand, within BPHYT_RS05390at 1240.250 kb on - strand, within BPHYT_RS05390at 1240.367 kb on - strandat 1240.441 kb on - strandat 1240.517 kb on - strand, within BPHYT_RS05395

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1
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1,238,818 - BPHYT_RS05385 0.85 -0.1
1,238,818 - BPHYT_RS05385 0.85 -0.3
1,238,902 - -0.9
1,239,100 + -0.3
1,239,101 - -0.8
1,239,160 + -1.4
1,239,213 - -0.1
1,239,771 - -0.5
1,240,065 + BPHYT_RS05390 0.48 +1.8
1,240,249 + BPHYT_RS05390 0.77 +0.5
1,240,250 - BPHYT_RS05390 0.77 -0.4
1,240,367 - +0.7
1,240,441 - +0.2
1,240,517 - BPHYT_RS05395 0.41 +0.7

Or see this region's nucleotide sequence