Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS03210 and BPHYT_RS03215 are separated by 837 nucleotides BPHYT_RS03215 and BPHYT_RS03220 are separated by 98 nucleotides
BPHYT_RS03210: BPHYT_RS03210 - cardiolipin synthetase, at 718,758 to 720,017
_RS03210
BPHYT_RS03215: BPHYT_RS03215 - TetR family transcriptional regulator, at 720,855 to 721,454
_RS03215
BPHYT_RS03220: BPHYT_RS03220 - Acyl-CoA dehydrogenase (EC 1.3.8.7) <small>(from data)</small>, at 721,553 to 723,340
_RS03220
Position (kb) 720
721
722 Strain fitness (log2 ratio) -1
0
1 at 719.965 kb on - strand at 720.656 kb on + strand at 720.770 kb on - strand at 720.770 kb on - strand at 720.838 kb on + strand at 720.838 kb on + strand at 721.164 kb on + strand, within BPHYT_RS03215 at 721.185 kb on + strand, within BPHYT_RS03215 at 721.186 kb on - strand, within BPHYT_RS03215 at 721.435 kb on - strand at 721.516 kb on + strand at 721.562 kb on + strand at 721.609 kb on + strand at 721.710 kb on + strand at 721.711 kb on - strand at 721.811 kb on + strand, within BPHYT_RS03220 at 721.811 kb on + strand, within BPHYT_RS03220 at 721.812 kb on - strand, within BPHYT_RS03220 at 721.812 kb on - strand, within BPHYT_RS03220 at 721.934 kb on + strand, within BPHYT_RS03220 at 721.935 kb on - strand, within BPHYT_RS03220 at 721.958 kb on + strand, within BPHYT_RS03220 at 722.003 kb on + strand, within BPHYT_RS03220
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 719,965 - -0.6 720,656 + -0.2 720,770 - +0.0 720,770 - -0.4 720,838 + -0.3 720,838 + -1.5 721,164 + BPHYT_RS03215 0.52 +1.4 721,185 + BPHYT_RS03215 0.55 -0.1 721,186 - BPHYT_RS03215 0.55 -0.9 721,435 - +0.5 721,516 + +0.2 721,562 + -0.9 721,609 + +0.2 721,710 + -0.2 721,711 - +0.7 721,811 + BPHYT_RS03220 0.14 +0.1 721,811 + BPHYT_RS03220 0.14 +0.4 721,812 - BPHYT_RS03220 0.14 -1.6 721,812 - BPHYT_RS03220 0.14 -1.0 721,934 + BPHYT_RS03220 0.21 -0.1 721,935 - BPHYT_RS03220 0.21 -0.8 721,958 + BPHYT_RS03220 0.23 +0.2 722,003 + BPHYT_RS03220 0.25 -1.1
Or see this region's nucleotide sequence