Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS02975 and BPHYT_RS02980 are separated by 135 nucleotides BPHYT_RS02980 and BPHYT_RS02985 are separated by 296 nucleotides BPHYT_RS02985 and BPHYT_RS02990 are separated by 202 nucleotides
BPHYT_RS02975: BPHYT_RS02975 - membrane protein, at 662,958 to 664,115
_RS02975
BPHYT_RS02980: BPHYT_RS02980 - single-stranded DNA-binding protein, at 664,251 to 664,790
_RS02980
BPHYT_RS02985: BPHYT_RS02985 - MaoC family dehydratase, at 665,087 to 665,587
_RS02985
BPHYT_RS02990: BPHYT_RS02990 - MFS transporter, at 665,790 to 667,175
_RS02990
Position (kb) 665
666 Strain fitness (log2 ratio) -2
-1
0
1
2 at 664.947 kb on + strand at 665.019 kb on + strand at 665.168 kb on + strand, within BPHYT_RS02985 at 665.169 kb on - strand, within BPHYT_RS02985 at 665.194 kb on + strand, within BPHYT_RS02985 at 665.541 kb on + strand at 665.542 kb on - strand at 665.545 kb on + strand at 665.908 kb on - strand at 665.908 kb on - strand at 665.908 kb on - strand at 666.137 kb on - strand, within BPHYT_RS02990 at 666.322 kb on - strand, within BPHYT_RS02990 at 666.323 kb on + strand, within BPHYT_RS02990 at 666.324 kb on - strand, within BPHYT_RS02990 at 666.324 kb on - strand, within BPHYT_RS02990 at 666.378 kb on - strand, within BPHYT_RS02990
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 664,947 + +0.6 665,019 + -1.9 665,168 + BPHYT_RS02985 0.16 -2.5 665,169 - BPHYT_RS02985 0.16 -0.9 665,194 + BPHYT_RS02985 0.21 -2.0 665,541 + -0.6 665,542 - -1.4 665,545 + +1.0 665,908 - -0.5 665,908 - -0.7 665,908 - +0.1 666,137 - BPHYT_RS02990 0.25 -0.2 666,322 - BPHYT_RS02990 0.38 -0.4 666,323 + BPHYT_RS02990 0.38 +1.2 666,324 - BPHYT_RS02990 0.39 +2.2 666,324 - BPHYT_RS02990 0.39 -0.5 666,378 - BPHYT_RS02990 0.42 -1.5
Or see this region's nucleotide sequence