Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00935 and BPHYT_RS00940 are separated by 205 nucleotides BPHYT_RS00940 and BPHYT_RS00945 are separated by 197 nucleotides
BPHYT_RS00935: BPHYT_RS00935 - integrase, at 213,540 to 215,057
_RS00935
BPHYT_RS00940: BPHYT_RS00940 - transposase, at 215,263 to 215,730
_RS00940
BPHYT_RS00945: BPHYT_RS00945 - hypothetical protein, at 215,928 to 216,449
_RS00945
Position (kb) 215
216 Strain fitness (log2 ratio) -2
-1
0
1
2 at 215.263 kb on + strand at 215.550 kb on - strand, within BPHYT_RS00940 at 215.550 kb on - strand, within BPHYT_RS00940 at 215.595 kb on + strand, within BPHYT_RS00940 at 215.621 kb on + strand, within BPHYT_RS00940 at 215.711 kb on + strand at 215.721 kb on - strand at 215.754 kb on + strand at 215.766 kb on + strand at 215.767 kb on - strand at 215.798 kb on + strand at 215.825 kb on - strand at 215.836 kb on - strand at 215.941 kb on - strand at 216.160 kb on - strand, within BPHYT_RS00945 at 216.160 kb on - strand, within BPHYT_RS00945 at 216.163 kb on - strand, within BPHYT_RS00945 at 216.163 kb on - strand, within BPHYT_RS00945 at 216.193 kb on - strand, within BPHYT_RS00945 at 216.193 kb on - strand, within BPHYT_RS00945 at 216.428 kb on + strand at 216.429 kb on - strand at 216.465 kb on + strand at 216.466 kb on - strand at 216.492 kb on + strand at 216.492 kb on + strand at 216.620 kb on + strand at 216.620 kb on + strand at 216.620 kb on + strand at 216.620 kb on + strand at 216.621 kb on - strand at 216.695 kb on + strand at 216.711 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 215,263 + +0.2 215,550 - BPHYT_RS00940 0.61 -2.0 215,550 - BPHYT_RS00940 0.61 -1.6 215,595 + BPHYT_RS00940 0.71 -0.5 215,621 + BPHYT_RS00940 0.76 -2.6 215,711 + -0.6 215,721 - -1.0 215,754 + -0.5 215,766 + -1.9 215,767 - -0.7 215,798 + -0.5 215,825 - +0.1 215,836 - -0.7 215,941 - -0.7 216,160 - BPHYT_RS00945 0.44 +0.3 216,160 - BPHYT_RS00945 0.44 +2.2 216,163 - BPHYT_RS00945 0.45 +0.2 216,163 - BPHYT_RS00945 0.45 -2.3 216,193 - BPHYT_RS00945 0.51 +0.0 216,193 - BPHYT_RS00945 0.51 -1.6 216,428 + -1.0 216,429 - +1.0 216,465 + -0.5 216,466 - +2.4 216,492 + +0.3 216,492 + +0.4 216,620 + -0.9 216,620 + +1.7 216,620 + -0.5 216,620 + -0.6 216,621 - -0.7 216,695 + -0.8 216,711 - +0.2
Or see this region's nucleotide sequence