Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00730 and BPHYT_RS00735 are separated by 251 nucleotides BPHYT_RS00735 and BPHYT_RS00740 overlap by 4 nucleotides BPHYT_RS00740 and BPHYT_RS00745 are separated by 290 nucleotides
BPHYT_RS00730: BPHYT_RS00730 - porin, at 169,621 to 170,655
_RS00730
BPHYT_RS00735: BPHYT_RS00735 - AlpA family transcriptional regulator, at 170,907 to 171,149
_RS00735
BPHYT_RS00740: BPHYT_RS00740 - hypothetical protein, at 171,146 to 171,472
_RS00740
BPHYT_RS00745: BPHYT_RS00745 - transcriptional regulator, at 171,763 to 172,167
_RS00745
Position (kb) 170
171
172 Strain fitness (log2 ratio) -2
-1
0
1
2 at 169.912 kb on + strand, within BPHYT_RS00730 at 170.140 kb on + strand, within BPHYT_RS00730 at 170.182 kb on + strand, within BPHYT_RS00730 at 170.224 kb on + strand, within BPHYT_RS00730 at 170.224 kb on + strand, within BPHYT_RS00730 at 170.224 kb on + strand, within BPHYT_RS00730 at 170.224 kb on + strand, within BPHYT_RS00730 at 170.224 kb on + strand, within BPHYT_RS00730 at 170.224 kb on + strand, within BPHYT_RS00730 at 170.225 kb on - strand, within BPHYT_RS00730 at 170.242 kb on + strand, within BPHYT_RS00730 at 170.383 kb on + strand, within BPHYT_RS00730 at 170.465 kb on + strand, within BPHYT_RS00730 at 170.465 kb on + strand, within BPHYT_RS00730 at 170.465 kb on + strand, within BPHYT_RS00730 at 170.465 kb on + strand, within BPHYT_RS00730 at 170.466 kb on - strand at 170.591 kb on - strand at 170.654 kb on - strand at 170.764 kb on + strand at 170.765 kb on - strand at 170.925 kb on - strand at 170.925 kb on - strand at 170.991 kb on + strand, within BPHYT_RS00735 at 170.992 kb on - strand, within BPHYT_RS00735 at 170.999 kb on + strand, within BPHYT_RS00735 at 171.000 kb on - strand, within BPHYT_RS00735 at 171.000 kb on - strand, within BPHYT_RS00735 at 171.299 kb on + strand, within BPHYT_RS00740 at 171.299 kb on + strand, within BPHYT_RS00740 at 171.300 kb on - strand, within BPHYT_RS00740 at 171.300 kb on - strand, within BPHYT_RS00740 at 171.529 kb on + strand at 171.530 kb on - strand at 171.530 kb on - strand at 171.533 kb on + strand at 171.533 kb on + strand at 171.664 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 169,912 + BPHYT_RS00730 0.28 -0.0 170,140 + BPHYT_RS00730 0.50 +1.6 170,182 + BPHYT_RS00730 0.54 -2.5 170,224 + BPHYT_RS00730 0.58 -0.6 170,224 + BPHYT_RS00730 0.58 +0.1 170,224 + BPHYT_RS00730 0.58 -0.2 170,224 + BPHYT_RS00730 0.58 -0.5 170,224 + BPHYT_RS00730 0.58 -0.2 170,224 + BPHYT_RS00730 0.58 -0.6 170,225 - BPHYT_RS00730 0.58 +0.3 170,242 + BPHYT_RS00730 0.60 +0.0 170,383 + BPHYT_RS00730 0.74 -0.6 170,465 + BPHYT_RS00730 0.82 -0.6 170,465 + BPHYT_RS00730 0.82 +0.1 170,465 + BPHYT_RS00730 0.82 -0.8 170,465 + BPHYT_RS00730 0.82 +0.3 170,466 - -1.6 170,591 - +2.4 170,654 - +1.6 170,764 + +0.6 170,765 - +0.1 170,925 - +0.4 170,925 - -1.3 170,991 + BPHYT_RS00735 0.35 -0.9 170,992 - BPHYT_RS00735 0.35 +0.1 170,999 + BPHYT_RS00735 0.38 +0.2 171,000 - BPHYT_RS00735 0.38 +0.5 171,000 - BPHYT_RS00735 0.38 -1.0 171,299 + BPHYT_RS00740 0.47 +0.5 171,299 + BPHYT_RS00740 0.47 +1.5 171,300 - BPHYT_RS00740 0.47 -0.7 171,300 - BPHYT_RS00740 0.47 -1.1 171,529 + +1.0 171,530 - +0.3 171,530 - +0.0 171,533 + +1.2 171,533 + +0.1 171,664 - -0.3
Or see this region's nucleotide sequence