Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00075 and BPHYT_RS00080 overlap by 8 nucleotides BPHYT_RS00080 and BPHYT_RS00085 are separated by 2 nucleotides BPHYT_RS00085 and BPHYT_RS00090 are separated by 204 nucleotides
BPHYT_RS00075: BPHYT_RS00075 - hypothetical protein, at 21,119 to 24,493
_RS00075
BPHYT_RS00080: BPHYT_RS00080 - hypothetical protein, at 24,486 to 25,133
_RS00080
BPHYT_RS00085: BPHYT_RS00085 - hypothetical protein, at 25,136 to 26,590
_RS00085
BPHYT_RS00090: BPHYT_RS00090 - reverse transcriptase, at 26,795 to 28,150
_RS00090
Position (kb) 25
26
27 Strain fitness (log2 ratio) -2
-1
0
1 at 24.138 kb on + strand, within BPHYT_RS00075 at 24.138 kb on + strand, within BPHYT_RS00075 at 24.139 kb on - strand, within BPHYT_RS00075 at 24.266 kb on - strand at 24.267 kb on + strand at 24.267 kb on + strand at 24.267 kb on + strand at 24.267 kb on + strand at 24.267 kb on + strand at 24.268 kb on - strand at 24.409 kb on + strand at 24.485 kb on - strand at 24.533 kb on - strand at 24.779 kb on + strand, within BPHYT_RS00080 at 24.779 kb on + strand, within BPHYT_RS00080 at 24.963 kb on + strand, within BPHYT_RS00080 at 24.988 kb on + strand, within BPHYT_RS00080 at 25.051 kb on + strand, within BPHYT_RS00080 at 25.137 kb on + strand at 25.138 kb on - strand at 25.247 kb on - strand at 25.255 kb on + strand at 25.304 kb on + strand, within BPHYT_RS00085 at 25.360 kb on + strand, within BPHYT_RS00085 at 25.448 kb on + strand, within BPHYT_RS00085 at 25.449 kb on - strand, within BPHYT_RS00085 at 25.488 kb on + strand, within BPHYT_RS00085 at 25.538 kb on - strand, within BPHYT_RS00085 at 25.940 kb on - strand, within BPHYT_RS00085 at 26.122 kb on + strand, within BPHYT_RS00085 at 26.122 kb on + strand, within BPHYT_RS00085 at 26.123 kb on - strand, within BPHYT_RS00085 at 26.123 kb on - strand, within BPHYT_RS00085 at 26.227 kb on + strand, within BPHYT_RS00085 at 26.313 kb on + strand, within BPHYT_RS00085 at 26.358 kb on - strand, within BPHYT_RS00085 at 26.361 kb on + strand, within BPHYT_RS00085 at 26.456 kb on + strand at 26.456 kb on + strand at 26.559 kb on - strand at 26.640 kb on - strand at 26.640 kb on - strand at 26.658 kb on + strand at 26.659 kb on - strand at 26.659 kb on - strand at 26.659 kb on - strand at 27.134 kb on + strand, within BPHYT_RS00090 at 27.547 kb on + strand, within BPHYT_RS00090
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 24,138 + BPHYT_RS00075 0.89 -1.8 24,138 + BPHYT_RS00075 0.89 +0.3 24,139 - BPHYT_RS00075 0.89 -0.1 24,266 - -1.1 24,267 + -0.5 24,267 + +0.5 24,267 + +0.7 24,267 + +0.6 24,267 + -0.7 24,268 - +0.3 24,409 + -0.4 24,485 - +1.7 24,533 - +1.1 24,779 + BPHYT_RS00080 0.45 -0.9 24,779 + BPHYT_RS00080 0.45 +0.4 24,963 + BPHYT_RS00080 0.74 -0.8 24,988 + BPHYT_RS00080 0.77 -1.1 25,051 + BPHYT_RS00080 0.87 -2.4 25,137 + +0.0 25,138 - +1.4 25,247 - -2.0 25,255 + -1.2 25,304 + BPHYT_RS00085 0.12 -0.9 25,360 + BPHYT_RS00085 0.15 -1.5 25,448 + BPHYT_RS00085 0.21 -0.4 25,449 - BPHYT_RS00085 0.22 -1.6 25,488 + BPHYT_RS00085 0.24 -0.4 25,538 - BPHYT_RS00085 0.28 -2.0 25,940 - BPHYT_RS00085 0.55 -0.7 26,122 + BPHYT_RS00085 0.68 -1.0 26,122 + BPHYT_RS00085 0.68 -0.4 26,123 - BPHYT_RS00085 0.68 +0.0 26,123 - BPHYT_RS00085 0.68 -0.3 26,227 + BPHYT_RS00085 0.75 -1.7 26,313 + BPHYT_RS00085 0.81 -0.8 26,358 - BPHYT_RS00085 0.84 +0.7 26,361 + BPHYT_RS00085 0.84 -1.2 26,456 + +0.5 26,456 + -0.4 26,559 - -0.0 26,640 - +1.0 26,640 - +0.2 26,658 + -0.6 26,659 - -1.3 26,659 - +0.4 26,659 - -0.1 27,134 + BPHYT_RS00090 0.25 -0.7 27,547 + BPHYT_RS00090 0.55 -1.1
Or see this region's nucleotide sequence