Experiment: 36hr PHYTO3 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00060 and BPHYT_RS00065 overlap by 4 nucleotides BPHYT_RS00065 and BPHYT_RS00070 are separated by 251 nucleotides BPHYT_RS00070 and BPHYT_RS00075 overlap by 4 nucleotides
BPHYT_RS00060: BPHYT_RS00060 - hypothetical protein, at 16,121 to 18,958
_RS00060
BPHYT_RS00065: BPHYT_RS00065 - hypothetical protein, at 18,955 to 19,692
_RS00065
BPHYT_RS00070: BPHYT_RS00070 - hypothetical protein, at 19,944 to 21,122
_RS00070
BPHYT_RS00075: BPHYT_RS00075 - hypothetical protein, at 21,119 to 24,493
_RS00075
Position (kb) 19
20
21
22 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 19.731 kb on - strand at 19.731 kb on - strand at 20.044 kb on + strand at 20.097 kb on - strand, within BPHYT_RS00070 at 20.355 kb on - strand, within BPHYT_RS00070 at 20.475 kb on - strand, within BPHYT_RS00070 at 20.478 kb on + strand, within BPHYT_RS00070 at 20.478 kb on + strand, within BPHYT_RS00070 at 20.478 kb on + strand, within BPHYT_RS00070 at 20.478 kb on + strand, within BPHYT_RS00070 at 20.594 kb on - strand, within BPHYT_RS00070 at 20.640 kb on + strand, within BPHYT_RS00070 at 20.640 kb on + strand, within BPHYT_RS00070 at 20.640 kb on + strand, within BPHYT_RS00070 at 20.641 kb on - strand, within BPHYT_RS00070 at 20.641 kb on - strand, within BPHYT_RS00070 at 20.642 kb on + strand, within BPHYT_RS00070 at 20.643 kb on - strand, within BPHYT_RS00070 at 20.643 kb on - strand, within BPHYT_RS00070 at 20.644 kb on + strand, within BPHYT_RS00070 at 20.644 kb on + strand, within BPHYT_RS00070 at 20.645 kb on - strand, within BPHYT_RS00070 at 20.645 kb on - strand, within BPHYT_RS00070 at 20.682 kb on + strand, within BPHYT_RS00070 at 20.682 kb on + strand, within BPHYT_RS00070 at 20.683 kb on - strand, within BPHYT_RS00070 at 20.683 kb on - strand, within BPHYT_RS00070 at 20.683 kb on - strand, within BPHYT_RS00070 at 20.683 kb on - strand, within BPHYT_RS00070 at 20.683 kb on - strand, within BPHYT_RS00070 at 20.683 kb on - strand, within BPHYT_RS00070 at 20.683 kb on - strand, within BPHYT_RS00070 at 20.876 kb on + strand, within BPHYT_RS00070 at 20.876 kb on + strand, within BPHYT_RS00070 at 21.041 kb on + strand at 21.042 kb on - strand at 21.222 kb on - strand at 21.240 kb on + strand at 21.240 kb on + strand at 21.241 kb on - strand at 21.241 kb on - strand at 21.378 kb on - strand at 21.430 kb on + strand at 21.430 kb on + strand at 21.430 kb on + strand at 21.431 kb on - strand at 21.431 kb on - strand at 21.431 kb on - strand at 21.433 kb on - strand at 21.480 kb on + strand, within BPHYT_RS00075 at 21.758 kb on - strand, within BPHYT_RS00075 at 21.759 kb on + strand, within BPHYT_RS00075 at 21.760 kb on - strand, within BPHYT_RS00075 at 21.774 kb on + strand, within BPHYT_RS00075 at 21.822 kb on - strand, within BPHYT_RS00075 at 21.898 kb on + strand, within BPHYT_RS00075
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO3 MOI 1 remove 19,731 - +0.8 19,731 - -0.7 20,044 + +0.7 20,097 - BPHYT_RS00070 0.13 +0.1 20,355 - BPHYT_RS00070 0.35 -0.0 20,475 - BPHYT_RS00070 0.45 -0.1 20,478 + BPHYT_RS00070 0.45 -0.2 20,478 + BPHYT_RS00070 0.45 -0.5 20,478 + BPHYT_RS00070 0.45 -0.2 20,478 + BPHYT_RS00070 0.45 -2.1 20,594 - BPHYT_RS00070 0.55 -0.7 20,640 + BPHYT_RS00070 0.59 +0.1 20,640 + BPHYT_RS00070 0.59 -0.0 20,640 + BPHYT_RS00070 0.59 +0.6 20,641 - BPHYT_RS00070 0.59 +1.0 20,641 - BPHYT_RS00070 0.59 +0.4 20,642 + BPHYT_RS00070 0.59 -1.1 20,643 - BPHYT_RS00070 0.59 -1.0 20,643 - BPHYT_RS00070 0.59 +0.7 20,644 + BPHYT_RS00070 0.59 -0.5 20,644 + BPHYT_RS00070 0.59 -1.3 20,645 - BPHYT_RS00070 0.59 +0.0 20,645 - BPHYT_RS00070 0.59 -0.5 20,682 + BPHYT_RS00070 0.63 +0.7 20,682 + BPHYT_RS00070 0.63 -0.6 20,683 - BPHYT_RS00070 0.63 -0.6 20,683 - BPHYT_RS00070 0.63 +0.4 20,683 - BPHYT_RS00070 0.63 +0.9 20,683 - BPHYT_RS00070 0.63 +0.3 20,683 - BPHYT_RS00070 0.63 -1.5 20,683 - BPHYT_RS00070 0.63 +0.1 20,683 - BPHYT_RS00070 0.63 -0.4 20,876 + BPHYT_RS00070 0.79 -0.1 20,876 + BPHYT_RS00070 0.79 +0.5 21,041 + +0.6 21,042 - +0.4 21,222 - +0.4 21,240 + -0.0 21,240 + -0.5 21,241 - +0.3 21,241 - -0.9 21,378 - -1.2 21,430 + -0.2 21,430 + +0.2 21,430 + +1.3 21,431 - -2.9 21,431 - -0.6 21,431 - -0.3 21,433 - -1.4 21,480 + BPHYT_RS00075 0.11 +2.0 21,758 - BPHYT_RS00075 0.19 -0.7 21,759 + BPHYT_RS00075 0.19 -1.1 21,760 - BPHYT_RS00075 0.19 +0.6 21,774 + BPHYT_RS00075 0.19 +2.4 21,822 - BPHYT_RS00075 0.21 +0.3 21,898 + BPHYT_RS00075 0.23 -2.2
Or see this region's nucleotide sequence