Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS35155

Experiment: 36hr PHYTO2 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS35150 and BPHYT_RS35155 are separated by 453 nucleotidesBPHYT_RS35155 and BPHYT_RS35160 are separated by 69 nucleotidesBPHYT_RS35160 and BPHYT_RS35165 overlap by 4 nucleotides BPHYT_RS35150: BPHYT_RS35150 - NADP-dependent aryl-alcohol dehydrogenase, at 3,489,625 to 3,490,620 _RS35150 BPHYT_RS35155: BPHYT_RS35155 - TonB-denpendent receptor, at 3,491,074 to 3,493,272 _RS35155 BPHYT_RS35160: BPHYT_RS35160 - PKHD-type hydroxylase, at 3,493,342 to 3,494,025 _RS35160 BPHYT_RS35165: BPHYT_RS35165 - peptidase, at 3,494,022 to 3,494,744 _RS35165 Position (kb) 3491 3492 3493 3494Strain fitness (log2 ratio) -2 -1 0 1 2at 3490.164 kb on + strand, within BPHYT_RS35150at 3490.253 kb on + strand, within BPHYT_RS35150at 3490.574 kb on - strandat 3490.674 kb on - strandat 3490.807 kb on - strandat 3490.850 kb on - strandat 3490.853 kb on + strandat 3490.854 kb on - strandat 3490.882 kb on + strandat 3490.882 kb on + strandat 3490.915 kb on + strandat 3490.916 kb on - strandat 3491.111 kb on + strandat 3491.112 kb on - strandat 3491.352 kb on + strand, within BPHYT_RS35155at 3491.353 kb on - strand, within BPHYT_RS35155at 3491.495 kb on - strand, within BPHYT_RS35155at 3491.689 kb on + strand, within BPHYT_RS35155at 3491.690 kb on - strand, within BPHYT_RS35155at 3491.727 kb on + strand, within BPHYT_RS35155at 3491.800 kb on + strand, within BPHYT_RS35155at 3491.878 kb on + strand, within BPHYT_RS35155at 3491.878 kb on + strand, within BPHYT_RS35155at 3491.878 kb on + strand, within BPHYT_RS35155at 3491.879 kb on - strand, within BPHYT_RS35155at 3491.879 kb on - strand, within BPHYT_RS35155at 3491.879 kb on - strand, within BPHYT_RS35155at 3491.947 kb on + strand, within BPHYT_RS35155at 3492.238 kb on + strand, within BPHYT_RS35155at 3492.238 kb on + strand, within BPHYT_RS35155at 3492.239 kb on - strand, within BPHYT_RS35155at 3492.330 kb on + strand, within BPHYT_RS35155at 3492.331 kb on - strand, within BPHYT_RS35155at 3492.592 kb on + strand, within BPHYT_RS35155at 3492.619 kb on + strand, within BPHYT_RS35155at 3492.620 kb on - strand, within BPHYT_RS35155at 3492.628 kb on + strand, within BPHYT_RS35155at 3492.628 kb on + strand, within BPHYT_RS35155at 3492.984 kb on + strand, within BPHYT_RS35155at 3493.007 kb on - strand, within BPHYT_RS35155at 3493.048 kb on + strand, within BPHYT_RS35155at 3493.049 kb on - strand, within BPHYT_RS35155at 3493.051 kb on - strand, within BPHYT_RS35155at 3493.099 kb on + strandat 3493.141 kb on + strandat 3493.181 kb on - strandat 3493.183 kb on + strandat 3493.186 kb on + strandat 3493.186 kb on + strandat 3493.187 kb on - strandat 3493.724 kb on - strand, within BPHYT_RS35160at 3493.990 kb on + strandat 3493.991 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 0.1
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3,490,164 + BPHYT_RS35150 0.54 +0.1
3,490,253 + BPHYT_RS35150 0.63 -0.1
3,490,574 - -1.8
3,490,674 - +0.1
3,490,807 - +0.5
3,490,850 - -0.1
3,490,853 + -0.8
3,490,854 - -0.3
3,490,882 + -0.9
3,490,882 + +1.2
3,490,915 + +0.4
3,490,916 - +0.5
3,491,111 + -0.8
3,491,112 - -0.2
3,491,352 + BPHYT_RS35155 0.13 +0.4
3,491,353 - BPHYT_RS35155 0.13 -0.6
3,491,495 - BPHYT_RS35155 0.19 +1.3
3,491,689 + BPHYT_RS35155 0.28 +0.7
3,491,690 - BPHYT_RS35155 0.28 -0.1
3,491,727 + BPHYT_RS35155 0.30 -0.6
3,491,800 + BPHYT_RS35155 0.33 -1.7
3,491,878 + BPHYT_RS35155 0.37 +0.2
3,491,878 + BPHYT_RS35155 0.37 -0.3
3,491,878 + BPHYT_RS35155 0.37 +0.3
3,491,879 - BPHYT_RS35155 0.37 -1.7
3,491,879 - BPHYT_RS35155 0.37 -0.6
3,491,879 - BPHYT_RS35155 0.37 -0.3
3,491,947 + BPHYT_RS35155 0.40 +0.6
3,492,238 + BPHYT_RS35155 0.53 -0.1
3,492,238 + BPHYT_RS35155 0.53 +0.2
3,492,239 - BPHYT_RS35155 0.53 +0.8
3,492,330 + BPHYT_RS35155 0.57 -0.9
3,492,331 - BPHYT_RS35155 0.57 -0.9
3,492,592 + BPHYT_RS35155 0.69 +2.4
3,492,619 + BPHYT_RS35155 0.70 +1.1
3,492,620 - BPHYT_RS35155 0.70 +1.1
3,492,628 + BPHYT_RS35155 0.71 +0.2
3,492,628 + BPHYT_RS35155 0.71 +0.4
3,492,984 + BPHYT_RS35155 0.87 +0.8
3,493,007 - BPHYT_RS35155 0.88 +0.4
3,493,048 + BPHYT_RS35155 0.90 +2.2
3,493,049 - BPHYT_RS35155 0.90 +1.2
3,493,051 - BPHYT_RS35155 0.90 +0.8
3,493,099 + +0.2
3,493,141 + +0.2
3,493,181 - +0.7
3,493,183 + +1.3
3,493,186 + -2.1
3,493,186 + -0.5
3,493,187 - -0.7
3,493,724 - BPHYT_RS35160 0.56 -0.1
3,493,990 + -0.3
3,493,991 - -0.3

Or see this region's nucleotide sequence