Experiment: 36hr PHYTO2 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS25485 and BPHYT_RS25490 overlap by 11 nucleotides BPHYT_RS25490 and BPHYT_RS25495 are separated by 32 nucleotides BPHYT_RS25495 and BPHYT_RS25500 are separated by 158 nucleotides BPHYT_RS25500 and BPHYT_RS25505 overlap by 4 nucleotides
BPHYT_RS25485: BPHYT_RS25485 - hypothetical protein, at 1,294,971 to 1,295,750
_RS25485
BPHYT_RS25490: BPHYT_RS25490 - hypothetical protein, at 1,295,740 to 1,296,663
_RS25490
BPHYT_RS25495: BPHYT_RS25495 - membrane protein, at 1,296,696 to 1,297,007
_RS25495
BPHYT_RS25500: BPHYT_RS25500 - hypothetical protein, at 1,297,166 to 1,297,807
_RS25500
BPHYT_RS25505: BPHYT_RS25505 - RNA polymerase sigma factor, at 1,297,804 to 1,298,349
_RS25505
Position (kb) 1296
1297
1298 Strain fitness (log2 ratio) -1
0
1
2 at 1295.848 kb on - strand, within BPHYT_RS25490 at 1295.982 kb on + strand, within BPHYT_RS25490 at 1296.035 kb on + strand, within BPHYT_RS25490 at 1296.084 kb on + strand, within BPHYT_RS25490 at 1296.495 kb on + strand, within BPHYT_RS25490 at 1296.498 kb on - strand, within BPHYT_RS25490 at 1296.654 kb on + strand at 1297.045 kb on + strand at 1297.046 kb on - strand at 1297.054 kb on + strand at 1297.222 kb on + strand at 1297.234 kb on - strand, within BPHYT_RS25500 at 1297.509 kb on - strand, within BPHYT_RS25500 at 1297.544 kb on - strand, within BPHYT_RS25500
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 0.1 remove 1,295,848 - BPHYT_RS25490 0.12 -0.0 1,295,982 + BPHYT_RS25490 0.26 +0.6 1,296,035 + BPHYT_RS25490 0.32 +1.3 1,296,084 + BPHYT_RS25490 0.37 +0.3 1,296,495 + BPHYT_RS25490 0.82 +1.1 1,296,498 - BPHYT_RS25490 0.82 +1.8 1,296,654 + +0.5 1,297,045 + -0.0 1,297,046 - -0.3 1,297,054 + +1.1 1,297,222 + +0.3 1,297,234 - BPHYT_RS25500 0.11 -0.7 1,297,509 - BPHYT_RS25500 0.53 -0.7 1,297,544 - BPHYT_RS25500 0.59 -0.9
Or see this region's nucleotide sequence