Experiment: 36hr PHYTO2 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS14660 and BPHYT_RS14665 are separated by 88 nucleotides BPHYT_RS14665 and BPHYT_RS14670 overlap by 4 nucleotides BPHYT_RS14670 and BPHYT_RS14675 are separated by 26 nucleotides
BPHYT_RS14660: BPHYT_RS14660 - hypothetical protein, at 3,341,217 to 3,341,714
_RS14660
BPHYT_RS14665: BPHYT_RS14665 - hypothetical protein, at 3,341,803 to 3,342,330
_RS14665
BPHYT_RS14670: BPHYT_RS14670 - hypothetical protein, at 3,342,327 to 3,342,962
_RS14670
BPHYT_RS14675: BPHYT_RS14675 - hypothetical protein, at 3,342,989 to 3,344,380
_RS14675
Position (kb)
3341
3342
3343 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3340.893 kb on + strand at 3340.899 kb on - strand at 3341.037 kb on - strand at 3341.063 kb on + strand at 3341.621 kb on - strand, within BPHYT_RS14660 at 3341.650 kb on + strand, within BPHYT_RS14660 at 3341.701 kb on - strand at 3341.788 kb on + strand at 3341.788 kb on + strand at 3341.788 kb on + strand at 3341.788 kb on + strand at 3341.788 kb on + strand at 3341.789 kb on - strand at 3341.789 kb on - strand at 3341.789 kb on - strand at 3342.258 kb on + strand, within BPHYT_RS14665 at 3342.258 kb on + strand, within BPHYT_RS14665 at 3342.259 kb on - strand, within BPHYT_RS14665 at 3342.769 kb on + strand, within BPHYT_RS14670 at 3342.970 kb on + strand at 3342.971 kb on - strand at 3343.065 kb on - strand at 3343.065 kb on - strand at 3343.065 kb on - strand at 3343.124 kb on + strand at 3343.124 kb on + strand at 3343.124 kb on + strand at 3343.125 kb on - strand at 3343.125 kb on - strand at 3343.220 kb on + strand, within BPHYT_RS14675 at 3343.221 kb on - strand, within BPHYT_RS14675 at 3343.222 kb on + strand, within BPHYT_RS14675 at 3343.247 kb on + strand, within BPHYT_RS14675 at 3343.248 kb on - strand, within BPHYT_RS14675 at 3343.258 kb on + strand, within BPHYT_RS14675 at 3343.258 kb on + strand, within BPHYT_RS14675 at 3343.258 kb on + strand, within BPHYT_RS14675 at 3343.259 kb on - strand, within BPHYT_RS14675 at 3343.259 kb on - strand, within BPHYT_RS14675 at 3343.259 kb on - strand, within BPHYT_RS14675 at 3343.259 kb on - strand, within BPHYT_RS14675
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 0.1 remove 3,340,893 + +0.7 3,340,899 - +0.7 3,341,037 - -0.1 3,341,063 + +0.7 3,341,621 - BPHYT_RS14660 0.81 -0.4 3,341,650 + BPHYT_RS14660 0.87 +0.2 3,341,701 - +0.2 3,341,788 + +0.2 3,341,788 + -0.2 3,341,788 + -1.3 3,341,788 + +0.6 3,341,788 + +0.7 3,341,789 - -0.2 3,341,789 - -2.0 3,341,789 - +0.3 3,342,258 + BPHYT_RS14665 0.86 -1.9 3,342,258 + BPHYT_RS14665 0.86 -1.6 3,342,259 - BPHYT_RS14665 0.86 +0.1 3,342,769 + BPHYT_RS14670 0.69 -0.8 3,342,970 + +0.3 3,342,971 - -1.9 3,343,065 - +0.7 3,343,065 - +0.3 3,343,065 - -1.0 3,343,124 + -0.6 3,343,124 + +0.9 3,343,124 + -0.4 3,343,125 - +0.3 3,343,125 - -1.7 3,343,220 + BPHYT_RS14675 0.17 +0.2 3,343,221 - BPHYT_RS14675 0.17 -0.1 3,343,222 + BPHYT_RS14675 0.17 -0.2 3,343,247 + BPHYT_RS14675 0.19 -0.1 3,343,248 - BPHYT_RS14675 0.19 +0.4 3,343,258 + BPHYT_RS14675 0.19 -1.8 3,343,258 + BPHYT_RS14675 0.19 -0.2 3,343,258 + BPHYT_RS14675 0.19 +2.0 3,343,259 - BPHYT_RS14675 0.19 -0.9 3,343,259 - BPHYT_RS14675 0.19 -0.1 3,343,259 - BPHYT_RS14675 0.19 -0.7 3,343,259 - BPHYT_RS14675 0.19 -1.3
Or see this region's nucleotide sequence