Experiment: 36hr PHYTO2 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS12495 and BPHYT_RS12500 are separated by 64 nucleotides BPHYT_RS12500 and BPHYT_RS12505 are separated by 127 nucleotides BPHYT_RS12505 and BPHYT_RS12510 are separated by 357 nucleotides
BPHYT_RS12495: BPHYT_RS12495 - helicase, at 2,850,040 to 2,852,307
_RS12495
BPHYT_RS12500: BPHYT_RS12500 - hypothetical protein, at 2,852,372 to 2,852,590
_RS12500
BPHYT_RS12505: BPHYT_RS12505 - lipoprotein, at 2,852,718 to 2,853,647
_RS12505
BPHYT_RS12510: BPHYT_RS12510 - RNA-binding protein, at 2,854,005 to 2,856,353
_RS12510
Position (kb)
2852
2853
2854 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2851.721 kb on + strand, within BPHYT_RS12495 at 2851.721 kb on + strand, within BPHYT_RS12495 at 2851.724 kb on + strand, within BPHYT_RS12495 at 2851.724 kb on + strand, within BPHYT_RS12495 at 2851.725 kb on - strand, within BPHYT_RS12495 at 2851.736 kb on + strand, within BPHYT_RS12495 at 2851.736 kb on + strand, within BPHYT_RS12495 at 2851.759 kb on - strand, within BPHYT_RS12495 at 2851.890 kb on - strand, within BPHYT_RS12495 at 2851.895 kb on + strand, within BPHYT_RS12495 at 2851.895 kb on + strand, within BPHYT_RS12495 at 2851.895 kb on + strand, within BPHYT_RS12495 at 2851.896 kb on - strand, within BPHYT_RS12495 at 2851.896 kb on - strand, within BPHYT_RS12495 at 2851.896 kb on - strand, within BPHYT_RS12495 at 2852.518 kb on + strand, within BPHYT_RS12500 at 2852.518 kb on + strand, within BPHYT_RS12500 at 2852.518 kb on + strand, within BPHYT_RS12500 at 2852.519 kb on - strand, within BPHYT_RS12500 at 2852.719 kb on + strand at 2852.719 kb on + strand at 2852.719 kb on + strand at 2853.813 kb on - strand at 2853.842 kb on + strand at 2853.872 kb on + strand at 2853.873 kb on - strand at 2853.954 kb on + strand at 2853.955 kb on - strand at 2854.005 kb on - strand at 2854.198 kb on - strand at 2854.198 kb on - strand at 2854.274 kb on - strand, within BPHYT_RS12510 at 2854.641 kb on + strand, within BPHYT_RS12510
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 0.1 remove 2,851,721 + BPHYT_RS12495 0.74 +0.6 2,851,721 + BPHYT_RS12495 0.74 -1.5 2,851,724 + BPHYT_RS12495 0.74 -2.2 2,851,724 + BPHYT_RS12495 0.74 +0.1 2,851,725 - BPHYT_RS12495 0.74 -0.1 2,851,736 + BPHYT_RS12495 0.75 -0.6 2,851,736 + BPHYT_RS12495 0.75 -1.8 2,851,759 - BPHYT_RS12495 0.76 -1.6 2,851,890 - BPHYT_RS12495 0.82 +0.3 2,851,895 + BPHYT_RS12495 0.82 +0.1 2,851,895 + BPHYT_RS12495 0.82 +0.2 2,851,895 + BPHYT_RS12495 0.82 +0.4 2,851,896 - BPHYT_RS12495 0.82 -0.2 2,851,896 - BPHYT_RS12495 0.82 +0.3 2,851,896 - BPHYT_RS12495 0.82 +0.9 2,852,518 + BPHYT_RS12500 0.67 +0.3 2,852,518 + BPHYT_RS12500 0.67 +0.5 2,852,518 + BPHYT_RS12500 0.67 +0.3 2,852,519 - BPHYT_RS12500 0.67 +1.7 2,852,719 + +1.5 2,852,719 + +2.3 2,852,719 + -0.2 2,853,813 - -0.4 2,853,842 + -0.6 2,853,872 + +0.3 2,853,873 - +1.9 2,853,954 + +0.5 2,853,955 - +0.7 2,854,005 - -1.3 2,854,198 - +0.3 2,854,198 - +0.7 2,854,274 - BPHYT_RS12510 0.11 +2.3 2,854,641 + BPHYT_RS12510 0.27 -1.2
Or see this region's nucleotide sequence