Experiment: 36hr PHYTO2 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS11330 and BPHYT_RS11335 are separated by 277 nucleotides BPHYT_RS11335 and BPHYT_RS11340 are separated by 200 nucleotides BPHYT_RS11340 and BPHYT_RS11345 overlap by 4 nucleotides BPHYT_RS11345 and BPHYT_RS11350 are separated by 115 nucleotides BPHYT_RS11350 and BPHYT_RS11355 are separated by 127 nucleotides
BPHYT_RS11330: BPHYT_RS11330 - phospholipase, at 2,573,764 to 2,575,101
_RS11330
BPHYT_RS11335: BPHYT_RS11335 - hypothetical protein, at 2,575,379 to 2,575,630
_RS11335
BPHYT_RS11340: BPHYT_RS11340 - hypothetical protein, at 2,575,831 to 2,576,061
_RS11340
BPHYT_RS11345: BPHYT_RS11345 - AraC family transcriptional regulator, at 2,576,058 to 2,576,960
_RS11345
BPHYT_RS11350: BPHYT_RS11350 - cupin, at 2,577,076 to 2,577,465
_RS11350
BPHYT_RS11355: BPHYT_RS11355 - LysR family transcriptional regulator, at 2,577,593 to 2,578,654
_RS11355
Position (kb)
2576
2577 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2575.244 kb on + strand at 2575.244 kb on + strand at 2575.245 kb on - strand at 2575.472 kb on - strand, within BPHYT_RS11335 at 2575.540 kb on + strand, within BPHYT_RS11335 at 2575.561 kb on + strand, within BPHYT_RS11335 at 2575.641 kb on + strand at 2575.642 kb on - strand at 2575.812 kb on + strand at 2575.812 kb on + strand at 2575.812 kb on + strand at 2575.813 kb on - strand at 2575.816 kb on - strand at 2575.816 kb on - strand at 2575.816 kb on - strand at 2575.910 kb on - strand, within BPHYT_RS11340 at 2575.996 kb on + strand, within BPHYT_RS11340 at 2576.157 kb on + strand, within BPHYT_RS11345 at 2576.247 kb on - strand, within BPHYT_RS11345 at 2576.247 kb on - strand, within BPHYT_RS11345 at 2576.250 kb on + strand, within BPHYT_RS11345 at 2576.251 kb on - strand, within BPHYT_RS11345 at 2576.251 kb on - strand, within BPHYT_RS11345 at 2576.265 kb on - strand, within BPHYT_RS11345 at 2576.403 kb on + strand, within BPHYT_RS11345 at 2576.403 kb on + strand, within BPHYT_RS11345 at 2576.403 kb on + strand, within BPHYT_RS11345 at 2576.404 kb on - strand, within BPHYT_RS11345 at 2576.606 kb on + strand, within BPHYT_RS11345 at 2576.653 kb on - strand, within BPHYT_RS11345 at 2576.773 kb on - strand, within BPHYT_RS11345 at 2577.780 kb on + strand, within BPHYT_RS11355 at 2577.781 kb on - strand, within BPHYT_RS11355 at 2577.781 kb on - strand, within BPHYT_RS11355 at 2577.928 kb on - strand, within BPHYT_RS11355
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 0.1 remove 2,575,244 + -1.0 2,575,244 + -0.2 2,575,245 - +0.2 2,575,472 - BPHYT_RS11335 0.37 +0.7 2,575,540 + BPHYT_RS11335 0.64 -1.1 2,575,561 + BPHYT_RS11335 0.72 +0.7 2,575,641 + -0.2 2,575,642 - -0.1 2,575,812 + -0.0 2,575,812 + +0.7 2,575,812 + -0.3 2,575,813 - -0.0 2,575,816 - +2.6 2,575,816 - +0.7 2,575,816 - +0.0 2,575,910 - BPHYT_RS11340 0.34 -0.1 2,575,996 + BPHYT_RS11340 0.71 +0.7 2,576,157 + BPHYT_RS11345 0.11 -0.7 2,576,247 - BPHYT_RS11345 0.21 -0.3 2,576,247 - BPHYT_RS11345 0.21 +0.6 2,576,250 + BPHYT_RS11345 0.21 -0.1 2,576,251 - BPHYT_RS11345 0.21 +0.3 2,576,251 - BPHYT_RS11345 0.21 +1.3 2,576,265 - BPHYT_RS11345 0.23 +0.5 2,576,403 + BPHYT_RS11345 0.38 -0.6 2,576,403 + BPHYT_RS11345 0.38 +0.5 2,576,403 + BPHYT_RS11345 0.38 -1.0 2,576,404 - BPHYT_RS11345 0.38 -1.2 2,576,606 + BPHYT_RS11345 0.61 -0.2 2,576,653 - BPHYT_RS11345 0.66 -0.0 2,576,773 - BPHYT_RS11345 0.79 -0.2 2,577,780 + BPHYT_RS11355 0.18 -2.4 2,577,781 - BPHYT_RS11355 0.18 -2.1 2,577,781 - BPHYT_RS11355 0.18 -2.9 2,577,928 - BPHYT_RS11355 0.32 -0.9
Or see this region's nucleotide sequence