Experiment: 36hr PHYTO2 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS09805 and BPHYT_RS09810 are separated by 5 nucleotides BPHYT_RS09810 and BPHYT_RS09815 are separated by 153 nucleotides BPHYT_RS09815 and BPHYT_RS09820 are separated by 121 nucleotides BPHYT_RS09820 and BPHYT_RS09825 are separated by 400 nucleotides
BPHYT_RS09805: BPHYT_RS09805 - hypothetical protein, at 2,227,794 to 2,228,591
_RS09805
BPHYT_RS09810: BPHYT_RS09810 - hypothetical protein, at 2,228,597 to 2,228,821
_RS09810
BPHYT_RS09815: BPHYT_RS09815 - malate synthase, at 2,228,975 to 2,231,149
_RS09815
BPHYT_RS09820: BPHYT_RS09820 - hypothetical protein, at 2,231,271 to 2,231,675
_RS09820
BPHYT_RS09825: BPHYT_RS09825 - Fe-S protein, at 2,232,076 to 2,232,951
_RS09825
Position (kb)
2228
2229
2230
2231
2232 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 2228.082 kb on + strand, within BPHYT_RS09805 at 2228.091 kb on + strand, within BPHYT_RS09805 at 2228.092 kb on - strand, within BPHYT_RS09805 at 2228.157 kb on + strand, within BPHYT_RS09805 at 2228.232 kb on + strand, within BPHYT_RS09805 at 2228.236 kb on - strand, within BPHYT_RS09805 at 2228.237 kb on + strand, within BPHYT_RS09805 at 2228.238 kb on - strand, within BPHYT_RS09805 at 2228.495 kb on - strand, within BPHYT_RS09805 at 2228.548 kb on - strand at 2228.548 kb on - strand at 2228.691 kb on - strand, within BPHYT_RS09810 at 2228.762 kb on + strand, within BPHYT_RS09810 at 2228.762 kb on + strand, within BPHYT_RS09810 at 2228.802 kb on - strand at 2229.984 kb on + strand, within BPHYT_RS09815 at 2230.571 kb on - strand, within BPHYT_RS09815 at 2230.879 kb on + strand, within BPHYT_RS09815 at 2230.881 kb on + strand, within BPHYT_RS09815 at 2230.881 kb on + strand, within BPHYT_RS09815 at 2231.678 kb on + strand at 2231.701 kb on + strand at 2232.095 kb on + strand at 2232.096 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 0.1 remove 2,228,082 + BPHYT_RS09805 0.36 +0.8 2,228,091 + BPHYT_RS09805 0.37 -1.4 2,228,092 - BPHYT_RS09805 0.37 +1.6 2,228,157 + BPHYT_RS09805 0.45 -0.4 2,228,232 + BPHYT_RS09805 0.55 +3.0 2,228,236 - BPHYT_RS09805 0.55 -0.2 2,228,237 + BPHYT_RS09805 0.56 +0.8 2,228,238 - BPHYT_RS09805 0.56 -1.0 2,228,495 - BPHYT_RS09805 0.88 -0.3 2,228,548 - +1.4 2,228,548 - -1.2 2,228,691 - BPHYT_RS09810 0.42 +1.4 2,228,762 + BPHYT_RS09810 0.73 +0.1 2,228,762 + BPHYT_RS09810 0.73 +0.1 2,228,802 - +1.1 2,229,984 + BPHYT_RS09815 0.46 +0.4 2,230,571 - BPHYT_RS09815 0.73 +0.4 2,230,879 + BPHYT_RS09815 0.88 -0.2 2,230,881 + BPHYT_RS09815 0.88 +0.2 2,230,881 + BPHYT_RS09815 0.88 +4.1 2,231,678 + -0.0 2,231,701 + -1.4 2,232,095 + -0.1 2,232,096 - -0.0
Or see this region's nucleotide sequence