Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS09815

Experiment: 36hr PHYTO2 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS09805 and BPHYT_RS09810 are separated by 5 nucleotidesBPHYT_RS09810 and BPHYT_RS09815 are separated by 153 nucleotidesBPHYT_RS09815 and BPHYT_RS09820 are separated by 121 nucleotidesBPHYT_RS09820 and BPHYT_RS09825 are separated by 400 nucleotides BPHYT_RS09805: BPHYT_RS09805 - hypothetical protein, at 2,227,794 to 2,228,591 _RS09805 BPHYT_RS09810: BPHYT_RS09810 - hypothetical protein, at 2,228,597 to 2,228,821 _RS09810 BPHYT_RS09815: BPHYT_RS09815 - malate synthase, at 2,228,975 to 2,231,149 _RS09815 BPHYT_RS09820: BPHYT_RS09820 - hypothetical protein, at 2,231,271 to 2,231,675 _RS09820 BPHYT_RS09825: BPHYT_RS09825 - Fe-S protein, at 2,232,076 to 2,232,951 _RS09825 Position (kb) 2228 2229 2230 2231 2232Strain fitness (log2 ratio) -1 0 1 2 3 4at 2228.082 kb on + strand, within BPHYT_RS09805at 2228.091 kb on + strand, within BPHYT_RS09805at 2228.092 kb on - strand, within BPHYT_RS09805at 2228.157 kb on + strand, within BPHYT_RS09805at 2228.232 kb on + strand, within BPHYT_RS09805at 2228.236 kb on - strand, within BPHYT_RS09805at 2228.237 kb on + strand, within BPHYT_RS09805at 2228.238 kb on - strand, within BPHYT_RS09805at 2228.495 kb on - strand, within BPHYT_RS09805at 2228.548 kb on - strandat 2228.548 kb on - strandat 2228.691 kb on - strand, within BPHYT_RS09810at 2228.762 kb on + strand, within BPHYT_RS09810at 2228.762 kb on + strand, within BPHYT_RS09810at 2228.802 kb on - strandat 2229.984 kb on + strand, within BPHYT_RS09815at 2230.571 kb on - strand, within BPHYT_RS09815at 2230.879 kb on + strand, within BPHYT_RS09815at 2230.881 kb on + strand, within BPHYT_RS09815at 2230.881 kb on + strand, within BPHYT_RS09815at 2231.678 kb on + strandat 2231.701 kb on + strandat 2232.095 kb on + strandat 2232.096 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 0.1
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2,228,082 + BPHYT_RS09805 0.36 +0.8
2,228,091 + BPHYT_RS09805 0.37 -1.4
2,228,092 - BPHYT_RS09805 0.37 +1.6
2,228,157 + BPHYT_RS09805 0.45 -0.4
2,228,232 + BPHYT_RS09805 0.55 +3.0
2,228,236 - BPHYT_RS09805 0.55 -0.2
2,228,237 + BPHYT_RS09805 0.56 +0.8
2,228,238 - BPHYT_RS09805 0.56 -1.0
2,228,495 - BPHYT_RS09805 0.88 -0.3
2,228,548 - +1.4
2,228,548 - -1.2
2,228,691 - BPHYT_RS09810 0.42 +1.4
2,228,762 + BPHYT_RS09810 0.73 +0.1
2,228,762 + BPHYT_RS09810 0.73 +0.1
2,228,802 - +1.1
2,229,984 + BPHYT_RS09815 0.46 +0.4
2,230,571 - BPHYT_RS09815 0.73 +0.4
2,230,879 + BPHYT_RS09815 0.88 -0.2
2,230,881 + BPHYT_RS09815 0.88 +0.2
2,230,881 + BPHYT_RS09815 0.88 +4.1
2,231,678 + -0.0
2,231,701 + -1.4
2,232,095 + -0.1
2,232,096 - -0.0

Or see this region's nucleotide sequence