Experiment: 36hr PHYTO2 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS08475 and BPHYT_RS08480 overlap by 4 nucleotides BPHYT_RS08480 and BPHYT_RS08485 are separated by 207 nucleotides BPHYT_RS08485 and BPHYT_RS08490 are separated by 36 nucleotides
BPHYT_RS08475: BPHYT_RS08475 - hypothetical protein, at 1,939,027 to 1,939,326
_RS08475
BPHYT_RS08480: BPHYT_RS08480 - transcriptional regulator, at 1,939,323 to 1,939,745
_RS08480
BPHYT_RS08485: BPHYT_RS08485 - undecaprenyl-phosphate glucose phosphotransferase, at 1,939,953 to 1,941,350
_RS08485
BPHYT_RS08490: BPHYT_RS08490 - Crp/Fnr family transcriptional regulator, at 1,941,387 to 1,942,121
_RS08490
Position (kb)
1939
1940
1941
1942 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1939.139 kb on - strand, within BPHYT_RS08475 at 1939.174 kb on + strand, within BPHYT_RS08475 at 1939.174 kb on + strand, within BPHYT_RS08475 at 1939.189 kb on + strand, within BPHYT_RS08475 at 1939.243 kb on + strand, within BPHYT_RS08475 at 1939.244 kb on - strand, within BPHYT_RS08475 at 1939.275 kb on - strand, within BPHYT_RS08475 at 1939.291 kb on + strand, within BPHYT_RS08475 at 1939.426 kb on - strand, within BPHYT_RS08480 at 1939.627 kb on + strand, within BPHYT_RS08480 at 1939.892 kb on + strand at 1940.030 kb on + strand at 1940.038 kb on + strand at 1940.153 kb on + strand, within BPHYT_RS08485 at 1940.153 kb on + strand, within BPHYT_RS08485 at 1940.178 kb on + strand, within BPHYT_RS08485 at 1940.696 kb on + strand, within BPHYT_RS08485 at 1941.009 kb on - strand, within BPHYT_RS08485 at 1941.182 kb on - strand, within BPHYT_RS08485 at 1941.319 kb on - strand at 1941.737 kb on - strand, within BPHYT_RS08490 at 1942.308 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 0.1 remove 1,939,139 - BPHYT_RS08475 0.37 -0.5 1,939,174 + BPHYT_RS08475 0.49 -0.9 1,939,174 + BPHYT_RS08475 0.49 +0.5 1,939,189 + BPHYT_RS08475 0.54 -0.3 1,939,243 + BPHYT_RS08475 0.72 +0.4 1,939,244 - BPHYT_RS08475 0.72 +0.1 1,939,275 - BPHYT_RS08475 0.83 -0.0 1,939,291 + BPHYT_RS08475 0.88 -1.4 1,939,426 - BPHYT_RS08480 0.24 +3.1 1,939,627 + BPHYT_RS08480 0.72 -0.8 1,939,892 + -0.4 1,940,030 + -0.6 1,940,038 + -0.5 1,940,153 + BPHYT_RS08485 0.14 -0.6 1,940,153 + BPHYT_RS08485 0.14 -0.8 1,940,178 + BPHYT_RS08485 0.16 +1.0 1,940,696 + BPHYT_RS08485 0.53 -0.5 1,941,009 - BPHYT_RS08485 0.76 +0.7 1,941,182 - BPHYT_RS08485 0.88 -0.3 1,941,319 - -0.4 1,941,737 - BPHYT_RS08490 0.48 -1.2 1,942,308 + -0.3
Or see this region's nucleotide sequence