Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS05525

Experiment: 36hr PHYTO2 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS05515 and BPHYT_RS05520 are separated by 220 nucleotidesBPHYT_RS05520 and BPHYT_RS05525 overlap by 17 nucleotidesBPHYT_RS05525 and BPHYT_RS05530 are separated by 91 nucleotidesBPHYT_RS05530 and BPHYT_RS05535 are separated by 211 nucleotides BPHYT_RS05515: BPHYT_RS05515 - hypothetical protein, at 1,266,556 to 1,267,086 _RS05515 BPHYT_RS05520: BPHYT_RS05520 - transcriptional regulator, at 1,267,307 to 1,268,032 _RS05520 BPHYT_RS05525: BPHYT_RS05525 - sensor histidine kinase, at 1,268,016 to 1,269,335 _RS05525 BPHYT_RS05530: BPHYT_RS05530 - hypothetical protein, at 1,269,427 to 1,269,693 _RS05530 BPHYT_RS05535: BPHYT_RS05535 - diguanylate cyclase, at 1,269,905 to 1,271,596 _RS05535 Position (kb) 1268 1269 1270Strain fitness (log2 ratio) -3 -2 -1 0 1at 1267.080 kb on + strandat 1267.163 kb on + strandat 1267.163 kb on + strandat 1267.163 kb on + strandat 1267.163 kb on + strandat 1267.189 kb on - strandat 1267.207 kb on - strandat 1267.220 kb on - strandat 1267.220 kb on - strandat 1267.313 kb on - strandat 1267.320 kb on + strandat 1267.320 kb on + strandat 1267.320 kb on + strandat 1267.321 kb on - strandat 1267.605 kb on - strand, within BPHYT_RS05520at 1267.881 kb on - strand, within BPHYT_RS05520at 1267.881 kb on - strand, within BPHYT_RS05520at 1267.881 kb on - strand, within BPHYT_RS05520at 1268.891 kb on - strand, within BPHYT_RS05525at 1268.919 kb on - strand, within BPHYT_RS05525at 1269.176 kb on + strand, within BPHYT_RS05525at 1269.336 kb on + strandat 1269.336 kb on + strandat 1269.336 kb on + strandat 1269.337 kb on - strandat 1269.337 kb on - strandat 1269.429 kb on - strandat 1269.444 kb on + strandat 1269.444 kb on + strandat 1269.445 kb on - strandat 1269.478 kb on - strand, within BPHYT_RS05530at 1269.509 kb on + strand, within BPHYT_RS05530at 1269.521 kb on + strand, within BPHYT_RS05530at 1269.522 kb on - strand, within BPHYT_RS05530at 1269.624 kb on - strand, within BPHYT_RS05530at 1269.731 kb on - strandat 1269.997 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 0.1
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1,267,080 + -0.6
1,267,163 + -0.5
1,267,163 + -1.4
1,267,163 + -0.3
1,267,163 + -0.4
1,267,189 - -0.4
1,267,207 - -1.5
1,267,220 - +0.2
1,267,220 - -0.2
1,267,313 - -0.2
1,267,320 + -0.4
1,267,320 + -0.4
1,267,320 + -1.2
1,267,321 - -0.2
1,267,605 - BPHYT_RS05520 0.41 -0.6
1,267,881 - BPHYT_RS05520 0.79 +0.6
1,267,881 - BPHYT_RS05520 0.79 +0.3
1,267,881 - BPHYT_RS05520 0.79 +0.6
1,268,891 - BPHYT_RS05525 0.66 -2.5
1,268,919 - BPHYT_RS05525 0.68 -0.4
1,269,176 + BPHYT_RS05525 0.88 -0.1
1,269,336 + +1.7
1,269,336 + +0.2
1,269,336 + -0.0
1,269,337 - -0.9
1,269,337 - +0.4
1,269,429 - -0.2
1,269,444 + -0.1
1,269,444 + -0.2
1,269,445 - -0.5
1,269,478 - BPHYT_RS05530 0.19 -0.8
1,269,509 + BPHYT_RS05530 0.31 -3.2
1,269,521 + BPHYT_RS05530 0.35 -1.2
1,269,522 - BPHYT_RS05530 0.36 -1.3
1,269,624 - BPHYT_RS05530 0.74 -0.0
1,269,731 - -0.9
1,269,997 - -0.1

Or see this region's nucleotide sequence