Experiment: 36hr PHYTO2 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS04190 and BPHYT_RS04195 are separated by 416 nucleotides BPHYT_RS04195 and BPHYT_RS04200 are separated by 55 nucleotides BPHYT_RS04200 and BPHYT_RS04205 are separated by 174 nucleotides
BPHYT_RS04190: BPHYT_RS04190 - AraC family transcriptional regulator, at 961,212 to 962,168
_RS04190
BPHYT_RS04195: BPHYT_RS04195 - molecular chaperone GroES, at 962,585 to 962,875
_RS04195
BPHYT_RS04200: BPHYT_RS04200 - molecular chaperone GroEL, at 962,931 to 964,571
_RS04200
BPHYT_RS04205: BPHYT_RS04205 - kinase, at 964,746 to 965,606
_RS04205
Position (kb)
962
963
964
965 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 962.167 kb on - strand at 962.281 kb on - strand at 962.327 kb on - strand at 962.327 kb on - strand at 962.394 kb on + strand at 962.394 kb on + strand at 962.394 kb on + strand at 962.395 kb on - strand at 962.395 kb on - strand at 962.403 kb on + strand at 962.434 kb on + strand at 962.434 kb on + strand at 962.434 kb on + strand at 962.434 kb on + strand at 962.435 kb on - strand at 964.744 kb on + strand at 964.838 kb on + strand, within BPHYT_RS04205 at 964.840 kb on + strand, within BPHYT_RS04205 at 964.841 kb on - strand, within BPHYT_RS04205 at 964.841 kb on - strand, within BPHYT_RS04205 at 964.841 kb on - strand, within BPHYT_RS04205 at 964.841 kb on - strand, within BPHYT_RS04205 at 964.888 kb on + strand, within BPHYT_RS04205 at 964.888 kb on + strand, within BPHYT_RS04205 at 964.889 kb on - strand, within BPHYT_RS04205 at 964.889 kb on - strand, within BPHYT_RS04205 at 964.889 kb on - strand, within BPHYT_RS04205 at 964.964 kb on + strand, within BPHYT_RS04205 at 964.964 kb on + strand, within BPHYT_RS04205 at 965.042 kb on + strand, within BPHYT_RS04205 at 965.042 kb on + strand, within BPHYT_RS04205 at 965.042 kb on + strand, within BPHYT_RS04205 at 965.083 kb on + strand, within BPHYT_RS04205
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 0.1 remove 962,167 - +0.0 962,281 - -0.4 962,327 - +0.5 962,327 - +0.6 962,394 + +0.0 962,394 + -1.2 962,394 + -2.9 962,395 - -0.4 962,395 - -0.3 962,403 + +0.1 962,434 + -1.1 962,434 + -1.7 962,434 + -0.7 962,434 + -0.7 962,435 - +0.7 964,744 + -0.0 964,838 + BPHYT_RS04205 0.11 +0.7 964,840 + BPHYT_RS04205 0.11 +0.5 964,841 - BPHYT_RS04205 0.11 -0.5 964,841 - BPHYT_RS04205 0.11 -1.9 964,841 - BPHYT_RS04205 0.11 -0.9 964,841 - BPHYT_RS04205 0.11 -0.9 964,888 + BPHYT_RS04205 0.16 -1.0 964,888 + BPHYT_RS04205 0.16 -1.6 964,889 - BPHYT_RS04205 0.17 -1.5 964,889 - BPHYT_RS04205 0.17 -1.3 964,889 - BPHYT_RS04205 0.17 -0.8 964,964 + BPHYT_RS04205 0.25 -0.8 964,964 + BPHYT_RS04205 0.25 +0.4 965,042 + BPHYT_RS04205 0.34 -1.1 965,042 + BPHYT_RS04205 0.34 -0.9 965,042 + BPHYT_RS04205 0.34 +0.4 965,083 + BPHYT_RS04205 0.39 -0.3
Or see this region's nucleotide sequence