Experiment: 36hr PHYTO2 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS00705 and BPHYT_RS00710 overlap by 14 nucleotides BPHYT_RS00710 and BPHYT_RS00715 are separated by 18 nucleotides BPHYT_RS00715 and BPHYT_RS00720 are separated by 54 nucleotides
BPHYT_RS00705: BPHYT_RS00705 - branched-chain amino acid ABC transporter substrate-binding protein, at 164,854 to 165,588
_RS00705
BPHYT_RS00710: BPHYT_RS00710 - branched-chain amino acid ABC transporter ATPase, at 165,575 to 166,318
_RS00710
BPHYT_RS00715: BPHYT_RS00715 - branched-chain amino acid ABC transporter substrate-binding protein, at 166,337 to 167,515
_RS00715
BPHYT_RS00720: BPHYT_RS00720 - amidase, at 167,570 to 168,997
_RS00720
Position (kb) 166
167
168 Strain fitness (log2 ratio) -1
0
1 at 165.515 kb on - strand at 165.553 kb on + strand at 165.553 kb on + strand at 165.554 kb on - strand at 165.912 kb on + strand, within BPHYT_RS00710 at 165.912 kb on + strand, within BPHYT_RS00710 at 165.920 kb on + strand, within BPHYT_RS00710 at 166.262 kb on + strand at 166.262 kb on + strand at 166.648 kb on + strand, within BPHYT_RS00715 at 167.157 kb on - strand, within BPHYT_RS00715 at 167.205 kb on - strand, within BPHYT_RS00715 at 167.206 kb on + strand, within BPHYT_RS00715 at 167.206 kb on + strand, within BPHYT_RS00715 at 167.216 kb on + strand, within BPHYT_RS00715 at 167.283 kb on - strand, within BPHYT_RS00715 at 167.517 kb on + strand at 167.517 kb on + strand at 167.517 kb on + strand at 167.659 kb on - strand at 168.492 kb on - strand, within BPHYT_RS00720 at 168.495 kb on - strand, within BPHYT_RS00720
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 0.1 remove 165,515 - -0.2 165,553 + +0.9 165,553 + -1.4 165,554 - -0.2 165,912 + BPHYT_RS00710 0.45 -0.3 165,912 + BPHYT_RS00710 0.45 +0.0 165,920 + BPHYT_RS00710 0.46 -1.5 166,262 + -1.4 166,262 + -0.1 166,648 + BPHYT_RS00715 0.26 -0.3 167,157 - BPHYT_RS00715 0.70 -0.7 167,205 - BPHYT_RS00715 0.74 +0.5 167,206 + BPHYT_RS00715 0.74 -0.2 167,206 + BPHYT_RS00715 0.74 -0.2 167,216 + BPHYT_RS00715 0.75 -0.4 167,283 - BPHYT_RS00715 0.80 -0.4 167,517 + +1.1 167,517 + +0.5 167,517 + -1.7 167,659 - +1.0 168,492 - BPHYT_RS00720 0.65 -1.3 168,495 - BPHYT_RS00720 0.65 -0.3
Or see this region's nucleotide sequence