Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS36355 and BPHYT_RS36360 are separated by 13 nucleotides BPHYT_RS36360 and BPHYT_RS36365 are separated by 153 nucleotides BPHYT_RS36365 and BPHYT_RS36370 are separated by 230 nucleotides
BPHYT_RS36355: BPHYT_RS36355 - hypothetical protein, at 84,525 to 84,875
_RS36355
BPHYT_RS36360: BPHYT_RS36360 - hypothetical protein, at 84,889 to 85,194
_RS36360
BPHYT_RS36365: BPHYT_RS36365 - hypothetical protein, at 85,348 to 85,650
_RS36365
BPHYT_RS36370: BPHYT_RS36370 - integrase, at 85,881 to 87,089
_RS36370
Position (kb) 85
86 Strain fitness (log2 ratio) -2
-1
0
1
2 at 84.423 kb on - strand at 84.801 kb on + strand, within BPHYT_RS36355 at 84.803 kb on - strand, within BPHYT_RS36355 at 84.980 kb on - strand, within BPHYT_RS36360 at 84.997 kb on + strand, within BPHYT_RS36360 at 85.099 kb on + strand, within BPHYT_RS36360 at 85.619 kb on + strand, within BPHYT_RS36365 at 85.675 kb on + strand at 85.990 kb on - strand at 86.260 kb on - strand, within BPHYT_RS36370 at 86.261 kb on + strand, within BPHYT_RS36370 at 86.317 kb on - strand, within BPHYT_RS36370 at 86.321 kb on - strand, within BPHYT_RS36370 at 86.430 kb on - strand, within BPHYT_RS36370 at 86.465 kb on + strand, within BPHYT_RS36370 at 86.465 kb on + strand, within BPHYT_RS36370
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 84,423 - +0.2 84,801 + BPHYT_RS36355 0.79 -0.7 84,803 - BPHYT_RS36355 0.79 +0.6 84,980 - BPHYT_RS36360 0.30 -1.3 84,997 + BPHYT_RS36360 0.35 -2.2 85,099 + BPHYT_RS36360 0.69 +0.8 85,619 + BPHYT_RS36365 0.89 +0.0 85,675 + +1.8 85,990 - +0.1 86,260 - BPHYT_RS36370 0.31 +0.2 86,261 + BPHYT_RS36370 0.31 +0.4 86,317 - BPHYT_RS36370 0.36 -1.3 86,321 - BPHYT_RS36370 0.36 -0.7 86,430 - BPHYT_RS36370 0.45 -0.4 86,465 + BPHYT_RS36370 0.48 +1.2 86,465 + BPHYT_RS36370 0.48 +0.3
Or see this region's nucleotide sequence