Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS35430 and BPHYT_RS35435 are separated by 186 nucleotides BPHYT_RS35435 and BPHYT_RS35440 are separated by 73 nucleotides BPHYT_RS35440 and BPHYT_RS35445 are separated by 29 nucleotides
BPHYT_RS35430: BPHYT_RS35430 - DSBA oxidoreductase, at 3,549,882 to 3,551,390
_RS35430
BPHYT_RS35435: BPHYT_RS35435 - glutathione S-transferase, at 3,551,577 to 3,552,254
_RS35435
BPHYT_RS35440: BPHYT_RS35440 - carboxymethylenebutenolidase, at 3,552,328 to 3,553,551
_RS35440
BPHYT_RS35445: BPHYT_RS35445 - 2-aminobenzoate-CoA ligase [EC: 6.2.1.32] (from data) , at 3,553,581 to 3,555,221
_RS35445
Position (kb)
3552
3553
3554 Strain fitness (log2 ratio)
-1
0
1
2 at 3551.489 kb on - strand at 3551.525 kb on + strand at 3551.525 kb on + strand at 3551.552 kb on + strand at 3551.630 kb on - strand at 3551.797 kb on + strand, within BPHYT_RS35435 at 3551.956 kb on + strand, within BPHYT_RS35435 at 3551.968 kb on + strand, within BPHYT_RS35435 at 3551.969 kb on - strand, within BPHYT_RS35435 at 3552.211 kb on - strand at 3552.211 kb on - strand at 3552.353 kb on - strand at 3552.451 kb on + strand, within BPHYT_RS35440 at 3552.471 kb on - strand, within BPHYT_RS35440 at 3552.471 kb on - strand, within BPHYT_RS35440 at 3552.474 kb on + strand, within BPHYT_RS35440 at 3552.474 kb on + strand, within BPHYT_RS35440 at 3552.474 kb on + strand at 3552.475 kb on - strand, within BPHYT_RS35440 at 3552.475 kb on - strand, within BPHYT_RS35440 at 3552.475 kb on - strand, within BPHYT_RS35440 at 3552.486 kb on - strand, within BPHYT_RS35440 at 3552.536 kb on + strand, within BPHYT_RS35440 at 3552.585 kb on + strand, within BPHYT_RS35440 at 3552.586 kb on - strand, within BPHYT_RS35440 at 3552.592 kb on - strand, within BPHYT_RS35440 at 3552.661 kb on - strand, within BPHYT_RS35440 at 3552.755 kb on + strand, within BPHYT_RS35440 at 3552.756 kb on - strand, within BPHYT_RS35440 at 3552.848 kb on + strand, within BPHYT_RS35440 at 3552.848 kb on + strand, within BPHYT_RS35440 at 3552.894 kb on - strand, within BPHYT_RS35440 at 3552.921 kb on - strand, within BPHYT_RS35440 at 3553.385 kb on + strand, within BPHYT_RS35440 at 3553.582 kb on + strand at 3553.671 kb on - strand at 3554.472 kb on - strand, within BPHYT_RS35445 at 3554.505 kb on + strand, within BPHYT_RS35445 at 3554.505 kb on + strand, within BPHYT_RS35445 at 3554.506 kb on - strand, within BPHYT_RS35445 at 3554.506 kb on - strand, within BPHYT_RS35445 at 3554.506 kb on - strand, within BPHYT_RS35445
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 3,551,489 - +0.4 3,551,525 + -1.3 3,551,525 + -0.1 3,551,552 + -0.2 3,551,630 - +0.2 3,551,797 + BPHYT_RS35435 0.32 -1.2 3,551,956 + BPHYT_RS35435 0.56 -0.7 3,551,968 + BPHYT_RS35435 0.58 -0.2 3,551,969 - BPHYT_RS35435 0.58 -0.8 3,552,211 - +1.1 3,552,211 - +1.3 3,552,353 - -0.1 3,552,451 + BPHYT_RS35440 0.10 +0.3 3,552,471 - BPHYT_RS35440 0.12 +0.7 3,552,471 - BPHYT_RS35440 0.12 +0.2 3,552,474 + BPHYT_RS35440 0.12 +0.4 3,552,474 + BPHYT_RS35440 0.12 -0.7 3,552,474 + +2.6 3,552,475 - BPHYT_RS35440 0.12 -0.5 3,552,475 - BPHYT_RS35440 0.12 +0.4 3,552,475 - BPHYT_RS35440 0.12 -0.5 3,552,486 - BPHYT_RS35440 0.13 -1.3 3,552,536 + BPHYT_RS35440 0.17 -1.0 3,552,585 + BPHYT_RS35440 0.21 +0.1 3,552,586 - BPHYT_RS35440 0.21 -0.5 3,552,592 - BPHYT_RS35440 0.22 +2.6 3,552,661 - BPHYT_RS35440 0.27 -0.0 3,552,755 + BPHYT_RS35440 0.35 +0.4 3,552,756 - BPHYT_RS35440 0.35 -1.0 3,552,848 + BPHYT_RS35440 0.42 +0.1 3,552,848 + BPHYT_RS35440 0.42 -0.8 3,552,894 - BPHYT_RS35440 0.46 -0.4 3,552,921 - BPHYT_RS35440 0.48 +1.7 3,553,385 + BPHYT_RS35440 0.86 +0.7 3,553,582 + +0.3 3,553,671 - +0.7 3,554,472 - BPHYT_RS35445 0.54 +0.0 3,554,505 + BPHYT_RS35445 0.56 -0.4 3,554,505 + BPHYT_RS35445 0.56 +0.0 3,554,506 - BPHYT_RS35445 0.56 -0.1 3,554,506 - BPHYT_RS35445 0.56 +0.2 3,554,506 - BPHYT_RS35445 0.56 -1.1
Or see this region's nucleotide sequence