Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS34085 and BPHYT_RS34090 are separated by 15 nucleotides BPHYT_RS34090 and BPHYT_RS34095 are separated by 119 nucleotides
BPHYT_RS34085: BPHYT_RS34085 - polyamine ABC transporter permease, at 3,244,965 to 3,245,804
_RS34085
BPHYT_RS34090: BPHYT_RS34090 - polyamine ABC transporter substrate-binding protein, at 3,245,820 to 3,247,097
_RS34090
BPHYT_RS34095: BPHYT_RS34095 - spermidine/putrescine ABC transporter substrate-binding protein, at 3,247,217 to 3,248,266
_RS34095
Position (kb)
3245
3246
3247
3248 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3244.852 kb on + strand at 3244.902 kb on + strand at 3244.962 kb on + strand at 3244.963 kb on - strand at 3244.974 kb on - strand at 3245.019 kb on + strand at 3245.019 kb on + strand at 3245.079 kb on - strand, within BPHYT_RS34085 at 3245.117 kb on + strand, within BPHYT_RS34085 at 3245.375 kb on + strand, within BPHYT_RS34085 at 3245.771 kb on + strand at 3245.772 kb on - strand at 3245.866 kb on + strand at 3245.867 kb on - strand at 3245.956 kb on + strand, within BPHYT_RS34090 at 3246.005 kb on - strand, within BPHYT_RS34090 at 3246.005 kb on - strand, within BPHYT_RS34090 at 3246.073 kb on + strand, within BPHYT_RS34090 at 3246.074 kb on - strand, within BPHYT_RS34090 at 3246.074 kb on - strand, within BPHYT_RS34090 at 3246.158 kb on + strand, within BPHYT_RS34090 at 3246.159 kb on - strand, within BPHYT_RS34090 at 3246.160 kb on + strand, within BPHYT_RS34090 at 3246.160 kb on + strand, within BPHYT_RS34090 at 3246.161 kb on - strand, within BPHYT_RS34090 at 3246.161 kb on - strand, within BPHYT_RS34090 at 3246.487 kb on + strand, within BPHYT_RS34090 at 3246.488 kb on - strand, within BPHYT_RS34090 at 3246.885 kb on - strand, within BPHYT_RS34090 at 3247.168 kb on - strand at 3247.401 kb on + strand, within BPHYT_RS34095 at 3247.440 kb on + strand, within BPHYT_RS34095 at 3247.501 kb on - strand, within BPHYT_RS34095 at 3247.570 kb on - strand, within BPHYT_RS34095 at 3247.762 kb on - strand, within BPHYT_RS34095 at 3247.762 kb on - strand, within BPHYT_RS34095
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 3,244,852 + +3.5 3,244,902 + +0.2 3,244,962 + -0.5 3,244,963 - +0.0 3,244,974 - -1.7 3,245,019 + -1.2 3,245,019 + -0.5 3,245,079 - BPHYT_RS34085 0.14 +1.6 3,245,117 + BPHYT_RS34085 0.18 +0.8 3,245,375 + BPHYT_RS34085 0.49 -1.5 3,245,771 + -1.5 3,245,772 - -0.8 3,245,866 + -0.2 3,245,867 - -0.9 3,245,956 + BPHYT_RS34090 0.11 +0.5 3,246,005 - BPHYT_RS34090 0.14 -1.4 3,246,005 - BPHYT_RS34090 0.14 +1.0 3,246,073 + BPHYT_RS34090 0.20 +1.1 3,246,074 - BPHYT_RS34090 0.20 -0.7 3,246,074 - BPHYT_RS34090 0.20 -0.2 3,246,158 + BPHYT_RS34090 0.26 +0.6 3,246,159 - BPHYT_RS34090 0.27 -0.1 3,246,160 + BPHYT_RS34090 0.27 -0.6 3,246,160 + BPHYT_RS34090 0.27 -1.0 3,246,161 - BPHYT_RS34090 0.27 -1.7 3,246,161 - BPHYT_RS34090 0.27 -1.2 3,246,487 + BPHYT_RS34090 0.52 -0.4 3,246,488 - BPHYT_RS34090 0.52 +0.8 3,246,885 - BPHYT_RS34090 0.83 -0.1 3,247,168 - -0.1 3,247,401 + BPHYT_RS34095 0.18 -0.5 3,247,440 + BPHYT_RS34095 0.21 -0.3 3,247,501 - BPHYT_RS34095 0.27 -0.9 3,247,570 - BPHYT_RS34095 0.34 +0.3 3,247,762 - BPHYT_RS34095 0.52 -0.3 3,247,762 - BPHYT_RS34095 0.52 -0.2
Or see this region's nucleotide sequence