Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS33235 and BPHYT_RS33240 are separated by 563 nucleotides BPHYT_RS33240 and BPHYT_RS33245 are separated by 161 nucleotides BPHYT_RS33245 and BPHYT_RS33250 are separated by 124 nucleotides
BPHYT_RS33235: BPHYT_RS33235 - potassium transporter Kup, at 3,037,238 to 3,039,163
_RS33235
BPHYT_RS33240: BPHYT_RS33240 - membrane protein, at 3,039,727 to 3,040,296
_RS33240
BPHYT_RS33245: BPHYT_RS33245 - hypothetical protein, at 3,040,458 to 3,040,778
_RS33245
BPHYT_RS33250: BPHYT_RS33250 - diguanylate phosphodiesterase, at 3,040,903 to 3,044,016
_RS33250
Position (kb)
3039
3040
3041 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3038.759 kb on + strand, within BPHYT_RS33235 at 3038.760 kb on - strand, within BPHYT_RS33235 at 3038.916 kb on + strand, within BPHYT_RS33235 at 3038.916 kb on + strand, within BPHYT_RS33235 at 3038.917 kb on - strand, within BPHYT_RS33235 at 3039.031 kb on - strand at 3039.068 kb on + strand at 3039.069 kb on - strand at 3039.353 kb on - strand at 3039.353 kb on - strand at 3039.437 kb on - strand at 3039.438 kb on + strand at 3039.523 kb on + strand at 3039.527 kb on + strand at 3039.916 kb on + strand, within BPHYT_RS33240 at 3039.917 kb on - strand, within BPHYT_RS33240 at 3040.124 kb on - strand, within BPHYT_RS33240 at 3040.370 kb on + strand at 3040.371 kb on - strand at 3040.686 kb on + strand, within BPHYT_RS33245 at 3040.686 kb on + strand, within BPHYT_RS33245 at 3040.687 kb on - strand, within BPHYT_RS33245 at 3040.758 kb on + strand at 3040.771 kb on - strand at 3040.829 kb on + strand at 3040.989 kb on - strand at 3041.000 kb on + strand at 3041.006 kb on + strand at 3041.006 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 3,038,759 + BPHYT_RS33235 0.79 -0.3 3,038,760 - BPHYT_RS33235 0.79 -1.7 3,038,916 + BPHYT_RS33235 0.87 -0.1 3,038,916 + BPHYT_RS33235 0.87 +1.5 3,038,917 - BPHYT_RS33235 0.87 -1.0 3,039,031 - +0.1 3,039,068 + +1.5 3,039,069 - -0.1 3,039,353 - -1.3 3,039,353 - -1.2 3,039,437 - -0.4 3,039,438 + +0.2 3,039,523 + +0.0 3,039,527 + +0.4 3,039,916 + BPHYT_RS33240 0.33 -1.7 3,039,917 - BPHYT_RS33240 0.33 -0.0 3,040,124 - BPHYT_RS33240 0.70 +1.9 3,040,370 + -0.2 3,040,371 - -2.6 3,040,686 + BPHYT_RS33245 0.71 +0.2 3,040,686 + BPHYT_RS33245 0.71 +0.8 3,040,687 - BPHYT_RS33245 0.71 -0.0 3,040,758 + +1.5 3,040,771 - -0.9 3,040,829 + -0.4 3,040,989 - +0.6 3,041,000 + +1.2 3,041,006 + -2.0 3,041,006 + -1.9
Or see this region's nucleotide sequence