Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS31520

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS31515 and BPHYT_RS31520 are separated by 595 nucleotidesBPHYT_RS31520 and BPHYT_RS31525 are separated by 477 nucleotides BPHYT_RS31515: BPHYT_RS31515 - agmatinase, at 2,623,781 to 2,624,755 _RS31515 BPHYT_RS31520: BPHYT_RS31520 - LysR family transcriptional regulator, at 2,625,351 to 2,626,265 _RS31520 BPHYT_RS31525: BPHYT_RS31525 - hypothetical protein, at 2,626,743 to 2,627,024 _RS31525 Position (kb) 2625 2626 2627Strain fitness (log2 ratio) -2 -1 0 1 2at 2624.409 kb on - strand, within BPHYT_RS31515at 2624.935 kb on - strandat 2625.100 kb on + strandat 2625.100 kb on + strandat 2625.552 kb on - strand, within BPHYT_RS31520at 2625.969 kb on + strand, within BPHYT_RS31520at 2625.970 kb on - strand, within BPHYT_RS31520at 2625.971 kb on + strand, within BPHYT_RS31520at 2625.972 kb on - strand, within BPHYT_RS31520at 2626.264 kb on - strandat 2626.281 kb on + strandat 2626.281 kb on + strandat 2626.334 kb on + strandat 2626.661 kb on + strandat 2626.807 kb on - strand, within BPHYT_RS31525at 2626.837 kb on + strand, within BPHYT_RS31525at 2626.943 kb on + strand, within BPHYT_RS31525at 2627.063 kb on + strandat 2627.218 kb on - strandat 2627.218 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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2,624,409 - BPHYT_RS31515 0.64 -0.1
2,624,935 - -1.1
2,625,100 + +0.5
2,625,100 + -2.1
2,625,552 - BPHYT_RS31520 0.22 +2.6
2,625,969 + BPHYT_RS31520 0.68 -0.6
2,625,970 - BPHYT_RS31520 0.68 +2.6
2,625,971 + BPHYT_RS31520 0.68 -1.1
2,625,972 - BPHYT_RS31520 0.68 -0.9
2,626,264 - +0.7
2,626,281 + -1.3
2,626,281 + +0.2
2,626,334 + -1.1
2,626,661 + +1.9
2,626,807 - BPHYT_RS31525 0.23 +1.3
2,626,837 + BPHYT_RS31525 0.33 +0.3
2,626,943 + BPHYT_RS31525 0.71 +1.6
2,627,063 + +1.0
2,627,218 - -1.1
2,627,218 - +1.6

Or see this region's nucleotide sequence