Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29675 and BPHYT_RS29680 are separated by 86 nucleotides BPHYT_RS29680 and BPHYT_RS29685 are separated by 171 nucleotides BPHYT_RS29685 and BPHYT_RS29690 are separated by 136 nucleotides BPHYT_RS29690 and BPHYT_RS29695 are separated by 23 nucleotides
BPHYT_RS29675: BPHYT_RS29675 - flagellar motor protein MotB, at 2,210,332 to 2,211,321
_RS29675
BPHYT_RS29680: BPHYT_RS29680 - hypothetical protein, at 2,211,408 to 2,212,091
_RS29680
BPHYT_RS29685: BPHYT_RS29685 - hypothetical protein, at 2,212,263 to 2,212,550
_RS29685
BPHYT_RS29690: BPHYT_RS29690 - exodeoxyribonuclease III, at 2,212,687 to 2,213,499
_RS29690
BPHYT_RS29695: BPHYT_RS29695 - hypothetical protein, at 2,213,523 to 2,213,741
_RS29695
Position (kb)
2212
2213 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2211.467 kb on - strand at 2211.698 kb on + strand, within BPHYT_RS29680 at 2211.723 kb on + strand, within BPHYT_RS29680 at 2211.723 kb on + strand, within BPHYT_RS29680 at 2211.723 kb on + strand, within BPHYT_RS29680 at 2211.769 kb on - strand, within BPHYT_RS29680 at 2212.044 kb on - strand at 2212.164 kb on + strand at 2212.165 kb on - strand at 2212.284 kb on - strand at 2213.018 kb on + strand, within BPHYT_RS29690 at 2213.019 kb on - strand, within BPHYT_RS29690 at 2213.084 kb on + strand, within BPHYT_RS29690 at 2213.086 kb on + strand, within BPHYT_RS29690 at 2213.166 kb on + strand, within BPHYT_RS29690 at 2213.174 kb on + strand, within BPHYT_RS29690
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 2,211,467 - -0.2 2,211,698 + BPHYT_RS29680 0.42 -1.0 2,211,723 + BPHYT_RS29680 0.46 -1.8 2,211,723 + BPHYT_RS29680 0.46 +0.6 2,211,723 + BPHYT_RS29680 0.46 -0.4 2,211,769 - BPHYT_RS29680 0.53 -0.9 2,212,044 - +0.2 2,212,164 + -0.5 2,212,165 - -0.4 2,212,284 - -2.5 2,213,018 + BPHYT_RS29690 0.41 -0.0 2,213,019 - BPHYT_RS29690 0.41 +0.3 2,213,084 + BPHYT_RS29690 0.49 -0.4 2,213,086 + BPHYT_RS29690 0.49 +3.6 2,213,166 + BPHYT_RS29690 0.59 -0.5 2,213,174 + BPHYT_RS29690 0.60 +0.3
Or see this region's nucleotide sequence