Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS29605 and BPHYT_RS29610 are separated by 155 nucleotides BPHYT_RS29610 and BPHYT_RS29615 are separated by 251 nucleotides BPHYT_RS29615 and BPHYT_RS29620 are separated by 294 nucleotides
BPHYT_RS29605: BPHYT_RS29605 - hypothetical protein, at 2,197,778 to 2,198,563
_RS29605
BPHYT_RS29610: BPHYT_RS29610 - cupin, at 2,198,719 to 2,199,030
_RS29610
BPHYT_RS29615: BPHYT_RS29615 - hypothetical protein, at 2,199,282 to 2,199,602
_RS29615
BPHYT_RS29620: BPHYT_RS29620 - hypothetical protein, at 2,199,897 to 2,200,214
_RS29620
Position (kb)
2198
2199
2200 Strain fitness (log2 ratio)
-2
-1
0
1 at 2197.725 kb on - strand at 2197.735 kb on + strand at 2197.737 kb on + strand at 2198.141 kb on + strand, within BPHYT_RS29605 at 2198.142 kb on - strand, within BPHYT_RS29605 at 2198.488 kb on + strand at 2198.489 kb on - strand at 2198.502 kb on - strand at 2198.613 kb on + strand at 2198.801 kb on + strand, within BPHYT_RS29610 at 2198.919 kb on + strand, within BPHYT_RS29610 at 2198.920 kb on - strand, within BPHYT_RS29610 at 2198.922 kb on - strand, within BPHYT_RS29610 at 2199.133 kb on + strand at 2199.133 kb on + strand at 2199.135 kb on + strand at 2199.136 kb on - strand at 2199.217 kb on - strand at 2199.218 kb on + strand at 2199.219 kb on - strand at 2199.219 kb on - strand at 2199.251 kb on - strand at 2199.251 kb on - strand at 2199.307 kb on + strand at 2199.308 kb on - strand at 2199.574 kb on + strand at 2199.575 kb on - strand at 2199.689 kb on + strand at 2199.689 kb on + strand at 2199.690 kb on - strand at 2199.898 kb on + strand at 2199.898 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 2,197,725 - +1.5 2,197,735 + -1.4 2,197,737 + +0.6 2,198,141 + BPHYT_RS29605 0.46 +0.8 2,198,142 - BPHYT_RS29605 0.46 +0.3 2,198,488 + -0.7 2,198,489 - +0.4 2,198,502 - -1.1 2,198,613 + -1.3 2,198,801 + BPHYT_RS29610 0.26 -0.4 2,198,919 + BPHYT_RS29610 0.64 -0.8 2,198,920 - BPHYT_RS29610 0.64 +0.4 2,198,922 - BPHYT_RS29610 0.65 -2.4 2,199,133 + -0.9 2,199,133 + -0.0 2,199,135 + -0.6 2,199,136 - -0.8 2,199,217 - +1.0 2,199,218 + +0.7 2,199,219 - -0.7 2,199,219 - +0.2 2,199,251 - -1.2 2,199,251 - +1.6 2,199,307 + -0.4 2,199,308 - -0.9 2,199,574 + -0.5 2,199,575 - +0.5 2,199,689 + -1.4 2,199,689 + -0.7 2,199,690 - -0.8 2,199,898 + -0.6 2,199,898 + +1.0
Or see this region's nucleotide sequence