Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS29025

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS29015 and BPHYT_RS29020 are separated by 113 nucleotidesBPHYT_RS29020 and BPHYT_RS29025 are separated by 410 nucleotidesBPHYT_RS29025 and BPHYT_RS29030 are separated by 377 nucleotidesBPHYT_RS29030 and BPHYT_RS29035 are separated by 69 nucleotides BPHYT_RS29015: BPHYT_RS29015 - RND transporter, at 2,066,861 to 2,068,432 _RS29015 BPHYT_RS29020: BPHYT_RS29020 - hypothetical protein, at 2,068,546 to 2,068,836 _RS29020 BPHYT_RS29025: BPHYT_RS29025 - hypothetical protein, at 2,069,247 to 2,069,582 _RS29025 BPHYT_RS29030: BPHYT_RS29030 - hypothetical protein, at 2,069,960 to 2,070,205 _RS29030 BPHYT_RS29035: BPHYT_RS29035 - arsenic transporter, at 2,070,275 to 2,071,525 _RS29035 Position (kb) 2069 2070Strain fitness (log2 ratio) -2 -1 0 1at 2068.601 kb on + strand, within BPHYT_RS29020at 2069.404 kb on - strand, within BPHYT_RS29025at 2069.642 kb on + strandat 2069.642 kb on + strandat 2069.695 kb on - strandat 2069.753 kb on - strandat 2069.967 kb on - strandat 2070.214 kb on + strandat 2070.225 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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2,068,601 + BPHYT_RS29020 0.19 -0.5
2,069,404 - BPHYT_RS29025 0.47 -1.3
2,069,642 + -0.1
2,069,642 + -1.9
2,069,695 - -0.5
2,069,753 - -1.8
2,069,967 - +0.7
2,070,214 + -1.3
2,070,225 + +0.3

Or see this region's nucleotide sequence