Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26685 and BPHYT_RS26690 are separated by 0 nucleotides BPHYT_RS26690 and BPHYT_RS26695 overlap by 13 nucleotides BPHYT_RS26695 and BPHYT_RS26700 are separated by 15 nucleotides BPHYT_RS26700 and BPHYT_RS26705 overlap by 4 nucleotides
BPHYT_RS26685: BPHYT_RS26685 - precorrin-8X methylmutase, at 1,555,879 to 1,556,505
_RS26685
BPHYT_RS26690: BPHYT_RS26690 - precorrin-2 C20-methyltransferase, at 1,556,506 to 1,557,237
_RS26690
BPHYT_RS26695: BPHYT_RS26695 - cobalamin biosynthesis protein CbiG, at 1,557,225 to 1,558,937
_RS26695
BPHYT_RS26700: BPHYT_RS26700 - hypothetical protein, at 1,558,953 to 1,559,564
_RS26700
BPHYT_RS26705: BPHYT_RS26705 - hypothetical protein, at 1,559,561 to 1,560,043
_RS26705
Position (kb)
1557
1558
1559 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1556.313 kb on + strand, within BPHYT_RS26685 at 1556.611 kb on + strand, within BPHYT_RS26690 at 1556.854 kb on + strand, within BPHYT_RS26690 at 1556.855 kb on - strand, within BPHYT_RS26690 at 1556.860 kb on + strand, within BPHYT_RS26690 at 1556.860 kb on + strand, within BPHYT_RS26690 at 1556.860 kb on + strand, within BPHYT_RS26690 at 1558.287 kb on + strand, within BPHYT_RS26695 at 1558.287 kb on + strand, within BPHYT_RS26695 at 1558.671 kb on + strand, within BPHYT_RS26695 at 1558.672 kb on - strand, within BPHYT_RS26695 at 1558.806 kb on - strand at 1558.811 kb on - strand at 1558.998 kb on + strand at 1558.998 kb on + strand at 1559.027 kb on + strand, within BPHYT_RS26700 at 1559.056 kb on + strand, within BPHYT_RS26700 at 1559.064 kb on + strand, within BPHYT_RS26700 at 1559.145 kb on + strand, within BPHYT_RS26700 at 1559.159 kb on - strand, within BPHYT_RS26700 at 1559.183 kb on - strand, within BPHYT_RS26700 at 1559.199 kb on - strand, within BPHYT_RS26700 at 1559.492 kb on - strand, within BPHYT_RS26700 at 1559.680 kb on + strand, within BPHYT_RS26705 at 1559.681 kb on - strand, within BPHYT_RS26705 at 1559.881 kb on - strand, within BPHYT_RS26705 at 1559.883 kb on + strand, within BPHYT_RS26705 at 1559.884 kb on - strand, within BPHYT_RS26705
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 1,556,313 + BPHYT_RS26685 0.69 +0.7 1,556,611 + BPHYT_RS26690 0.14 -1.8 1,556,854 + BPHYT_RS26690 0.48 +0.3 1,556,855 - BPHYT_RS26690 0.48 -1.4 1,556,860 + BPHYT_RS26690 0.48 -0.6 1,556,860 + BPHYT_RS26690 0.48 -2.3 1,556,860 + BPHYT_RS26690 0.48 +1.6 1,558,287 + BPHYT_RS26695 0.62 -0.4 1,558,287 + BPHYT_RS26695 0.62 +2.6 1,558,671 + BPHYT_RS26695 0.84 -0.1 1,558,672 - BPHYT_RS26695 0.84 +0.9 1,558,806 - -0.3 1,558,811 - -1.0 1,558,998 + +0.5 1,558,998 + -0.2 1,559,027 + BPHYT_RS26700 0.12 -0.2 1,559,056 + BPHYT_RS26700 0.17 +0.3 1,559,064 + BPHYT_RS26700 0.18 -1.1 1,559,145 + BPHYT_RS26700 0.31 -0.1 1,559,159 - BPHYT_RS26700 0.34 +1.3 1,559,183 - BPHYT_RS26700 0.38 +0.8 1,559,199 - BPHYT_RS26700 0.40 +2.2 1,559,492 - BPHYT_RS26700 0.88 -0.4 1,559,680 + BPHYT_RS26705 0.25 -0.4 1,559,681 - BPHYT_RS26705 0.25 -0.4 1,559,881 - BPHYT_RS26705 0.66 -0.2 1,559,883 + BPHYT_RS26705 0.67 +1.2 1,559,884 - BPHYT_RS26705 0.67 -0.2
Or see this region's nucleotide sequence