Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26495 and BPHYT_RS26500 are separated by 39 nucleotides BPHYT_RS26500 and BPHYT_RS26505 overlap by 4 nucleotides BPHYT_RS26505 and BPHYT_RS26510 are separated by 160 nucleotides BPHYT_RS26510 and BPHYT_RS26515 are separated by 24 nucleotides BPHYT_RS26515 and BPHYT_RS26520 overlap by 2 nucleotides BPHYT_RS26520 and BPHYT_RS26525 are separated by 26 nucleotides
BPHYT_RS26495: BPHYT_RS26495 - glutathione S-transferase, at 1,515,044 to 1,515,682
_RS26495
BPHYT_RS26500: BPHYT_RS26500 - ATPase, at 1,515,722 to 1,516,171
_RS26500
BPHYT_RS26505: BPHYT_RS26505 - ArsR family transcriptional regulator, at 1,516,168 to 1,516,503
_RS26505
BPHYT_RS26510: BPHYT_RS26510 - transcriptional regulator, at 1,516,664 to 1,517,569
_RS26510
BPHYT_RS26515: BPHYT_RS26515 - HAD family hydrolase, at 1,517,594 to 1,518,208
_RS26515
BPHYT_RS26520: BPHYT_RS26520 - hypothetical protein, at 1,518,207 to 1,518,386
_RS26520
BPHYT_RS26525: BPHYT_RS26525 - amino acid ABC transporter substrate-binding protein, at 1,518,413 to 1,519,564
_RS26525
Position (kb)
1516
1517
1518 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1515.670 kb on - strand at 1516.857 kb on - strand, within BPHYT_RS26510 at 1516.865 kb on + strand, within BPHYT_RS26510 at 1517.175 kb on - strand, within BPHYT_RS26510 at 1517.595 kb on + strand at 1517.595 kb on + strand at 1517.875 kb on + strand, within BPHYT_RS26515 at 1517.875 kb on + strand, within BPHYT_RS26515
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 1,515,670 - -1.8 1,516,857 - BPHYT_RS26510 0.21 -1.2 1,516,865 + BPHYT_RS26510 0.22 -0.9 1,517,175 - BPHYT_RS26510 0.56 +2.6 1,517,595 + -0.2 1,517,595 + -0.0 1,517,875 + BPHYT_RS26515 0.46 +0.4 1,517,875 + BPHYT_RS26515 0.46 +1.0
Or see this region's nucleotide sequence