Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26230 and BPHYT_RS26235 are separated by 172 nucleotides BPHYT_RS26235 and BPHYT_RS26240 are separated by 145 nucleotides BPHYT_RS26240 and BPHYT_RS26245 are separated by 259 nucleotides BPHYT_RS26245 and BPHYT_RS26250 are separated by 51 nucleotides
BPHYT_RS26230: BPHYT_RS26230 - MFS transporter, at 1,455,995 to 1,457,329
_RS26230
BPHYT_RS26235: BPHYT_RS26235 - hypothetical protein, at 1,457,502 to 1,458,038
_RS26235
BPHYT_RS26240: BPHYT_RS26240 - hypothetical protein, at 1,458,184 to 1,458,480
_RS26240
BPHYT_RS26245: BPHYT_RS26245 - thioredoxin, at 1,458,740 to 1,459,060
_RS26245
BPHYT_RS26250: BPHYT_RS26250 - hypothetical protein, at 1,459,112 to 1,459,531
_RS26250
Position (kb)
1458
1459 Strain fitness (log2 ratio)
-1
0
1
2 at 1457.546 kb on - strand at 1457.546 kb on - strand at 1457.552 kb on - strand at 1457.552 kb on - strand at 1457.560 kb on + strand, within BPHYT_RS26235 at 1458.151 kb on + strand at 1458.240 kb on - strand, within BPHYT_RS26240 at 1458.240 kb on - strand, within BPHYT_RS26240 at 1458.240 kb on - strand, within BPHYT_RS26240 at 1458.240 kb on - strand, within BPHYT_RS26240 at 1458.394 kb on + strand, within BPHYT_RS26240 at 1458.465 kb on - strand at 1458.561 kb on + strand at 1458.659 kb on - strand at 1458.759 kb on - strand at 1459.059 kb on - strand at 1459.085 kb on - strand at 1459.085 kb on - strand at 1459.244 kb on - strand, within BPHYT_RS26250 at 1459.244 kb on - strand, within BPHYT_RS26250 at 1459.308 kb on - strand, within BPHYT_RS26250 at 1459.464 kb on + strand, within BPHYT_RS26250 at 1459.464 kb on + strand, within BPHYT_RS26250 at 1459.465 kb on - strand, within BPHYT_RS26250 at 1459.465 kb on - strand, within BPHYT_RS26250 at 1459.467 kb on - strand, within BPHYT_RS26250
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 1,457,546 - +1.0 1,457,546 - -0.7 1,457,552 - -0.6 1,457,552 - -0.7 1,457,560 + BPHYT_RS26235 0.11 +2.2 1,458,151 + +0.5 1,458,240 - BPHYT_RS26240 0.19 -0.1 1,458,240 - BPHYT_RS26240 0.19 -0.7 1,458,240 - BPHYT_RS26240 0.19 -0.8 1,458,240 - BPHYT_RS26240 0.19 -0.0 1,458,394 + BPHYT_RS26240 0.71 +1.0 1,458,465 - -1.4 1,458,561 + -0.5 1,458,659 - +0.3 1,458,759 - -0.3 1,459,059 - -0.9 1,459,085 - +0.5 1,459,085 - +0.7 1,459,244 - BPHYT_RS26250 0.31 -0.5 1,459,244 - BPHYT_RS26250 0.31 -0.4 1,459,308 - BPHYT_RS26250 0.47 -1.1 1,459,464 + BPHYT_RS26250 0.84 -1.6 1,459,464 + BPHYT_RS26250 0.84 -0.5 1,459,465 - BPHYT_RS26250 0.84 -1.5 1,459,465 - BPHYT_RS26250 0.84 -0.7 1,459,467 - BPHYT_RS26250 0.85 -0.3
Or see this region's nucleotide sequence