Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS26025 and BPHYT_RS26030 overlap by 10 nucleotides BPHYT_RS26030 and BPHYT_RS26035 overlap by 4 nucleotides BPHYT_RS26035 and BPHYT_RS26040 are separated by 2 nucleotides BPHYT_RS26040 and BPHYT_RS26045 are separated by 61 nucleotides
BPHYT_RS26025: BPHYT_RS26025 - hypothetical protein, at 1,414,729 to 1,415,124
_RS26025
BPHYT_RS26030: BPHYT_RS26030 - cysteine hydrolase, at 1,415,115 to 1,415,810
_RS26030
BPHYT_RS26035: BPHYT_RS26035 - ABC transporter ATP-binding protein, at 1,415,807 to 1,417,417
_RS26035
BPHYT_RS26040: BPHYT_RS26040 - cysteine hydrolase, at 1,417,420 to 1,418,112
_RS26040
BPHYT_RS26045: BPHYT_RS26045 - ABC transporter permease, at 1,418,174 to 1,419,091
_RS26045
Position (kb)
1415
1416
1417
1418 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1415.070 kb on - strand, within BPHYT_RS26025 at 1415.236 kb on + strand, within BPHYT_RS26030 at 1415.588 kb on + strand, within BPHYT_RS26030 at 1415.588 kb on + strand, within BPHYT_RS26030 at 1416.918 kb on - strand, within BPHYT_RS26035 at 1416.939 kb on + strand, within BPHYT_RS26035 at 1416.940 kb on - strand, within BPHYT_RS26035 at 1417.417 kb on + strand at 1417.418 kb on - strand at 1417.489 kb on - strand at 1417.995 kb on - strand, within BPHYT_RS26040 at 1418.118 kb on - strand at 1418.278 kb on - strand, within BPHYT_RS26045 at 1418.278 kb on - strand, within BPHYT_RS26045 at 1418.284 kb on - strand, within BPHYT_RS26045
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 1,415,070 - BPHYT_RS26025 0.86 +1.0 1,415,236 + BPHYT_RS26030 0.17 +2.5 1,415,588 + BPHYT_RS26030 0.68 -2.1 1,415,588 + BPHYT_RS26030 0.68 +0.5 1,416,918 - BPHYT_RS26035 0.69 -1.4 1,416,939 + BPHYT_RS26035 0.70 -1.1 1,416,940 - BPHYT_RS26035 0.70 +0.5 1,417,417 + +0.9 1,417,418 - +0.5 1,417,489 - -0.7 1,417,995 - BPHYT_RS26040 0.83 +0.9 1,418,118 - +0.4 1,418,278 - BPHYT_RS26045 0.11 +0.2 1,418,278 - BPHYT_RS26045 0.11 +0.3 1,418,284 - BPHYT_RS26045 0.12 +0.0
Or see this region's nucleotide sequence