Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS23915

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS23905 and BPHYT_RS23910 overlap by 1 nucleotidesBPHYT_RS23910 and BPHYT_RS23915 overlap by 1 nucleotidesBPHYT_RS23915 and BPHYT_RS23920 overlap by 4 nucleotidesBPHYT_RS23920 and BPHYT_RS23925 overlap by 4 nucleotides BPHYT_RS23905: BPHYT_RS23905 - malonate decarboxylase subunit epsilon, at 929,769 to 930,701 _RS23905 BPHYT_RS23910: BPHYT_RS23910 - phosphoribosyl-dephospho-CoA transferase, at 930,701 to 931,399 _RS23910 BPHYT_RS23915: BPHYT_RS23915 - malonate decarboxylase subunit gamma, at 931,399 to 932,217 _RS23915 BPHYT_RS23920: BPHYT_RS23920 - malonate decarboxylase subunit beta, at 932,214 to 933,098 _RS23920 BPHYT_RS23925: BPHYT_RS23925 - malonate decarboxylase subunit delta, at 933,095 to 933,409 _RS23925 Position (kb) 931 932 933Strain fitness (log2 ratio) -2 -1 0 1 2at 930.576 kb on - strand, within BPHYT_RS23905at 931.139 kb on + strand, within BPHYT_RS23910at 932.572 kb on + strand, within BPHYT_RS23920at 932.573 kb on - strand, within BPHYT_RS23920at 933.093 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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930,576 - BPHYT_RS23905 0.86 -2.3
931,139 + BPHYT_RS23910 0.63 +2.5
932,572 + BPHYT_RS23920 0.40 -0.8
932,573 - BPHYT_RS23920 0.41 -1.1
933,093 - -1.8

Or see this region's nucleotide sequence