Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS23330

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS23320 and BPHYT_RS23325 are separated by 8 nucleotidesBPHYT_RS23325 and BPHYT_RS23330 are separated by 400 nucleotidesBPHYT_RS23330 and BPHYT_RS23335 are separated by 8 nucleotides BPHYT_RS23320: BPHYT_RS23320 - hypothetical protein, at 793,166 to 793,630 _RS23320 BPHYT_RS23325: BPHYT_RS23325 - ribonuclease, at 793,639 to 794,094 _RS23325 BPHYT_RS23330: BPHYT_RS23330 - hypothetical protein, at 794,495 to 794,680 _RS23330 BPHYT_RS23335: BPHYT_RS23335 - aspartate racemase, at 794,689 to 795,390 _RS23335 Position (kb) 794 795Strain fitness (log2 ratio) -2 -1 0 1at 793.617 kb on + strandat 793.641 kb on - strandat 793.683 kb on - strandat 793.721 kb on - strand, within BPHYT_RS23325at 793.721 kb on - strand, within BPHYT_RS23325at 793.769 kb on + strand, within BPHYT_RS23325at 793.770 kb on - strand, within BPHYT_RS23325at 794.138 kb on - strandat 794.138 kb on - strandat 794.144 kb on + strandat 794.387 kb on + strandat 794.405 kb on + strandat 795.513 kb on + strandat 795.570 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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793,617 + -0.8
793,641 - +0.4
793,683 - -0.2
793,721 - BPHYT_RS23325 0.18 -0.6
793,721 - BPHYT_RS23325 0.18 +1.0
793,769 + BPHYT_RS23325 0.29 +0.2
793,770 - BPHYT_RS23325 0.29 +0.2
794,138 - -0.2
794,138 - -0.4
794,144 + -0.3
794,387 + -2.1
794,405 + -0.1
795,513 + -0.0
795,570 - +0.2

Or see this region's nucleotide sequence