Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS23015

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS23010 and BPHYT_RS23015 are separated by 59 nucleotidesBPHYT_RS23015 and BPHYT_RS23020 are separated by 193 nucleotides BPHYT_RS23010: BPHYT_RS23010 - transcriptional regulator, at 722,300 to 723,157 _RS23010 BPHYT_RS23015: BPHYT_RS23015 - porin, at 723,217 to 724,410 _RS23015 BPHYT_RS23020: BPHYT_RS23020 - alcohol dehydrogenase, at 724,604 to 725,959 _RS23020 Position (kb) 723 724 725Strain fitness (log2 ratio) -2 -1 0 1at 722.482 kb on + strand, within BPHYT_RS23010at 722.518 kb on + strand, within BPHYT_RS23010at 723.473 kb on + strand, within BPHYT_RS23015at 723.473 kb on + strand, within BPHYT_RS23015at 723.902 kb on + strand, within BPHYT_RS23015at 723.903 kb on - strand, within BPHYT_RS23015at 724.733 kb on + strandat 724.733 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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722,482 + BPHYT_RS23010 0.21 -0.5
722,518 + BPHYT_RS23010 0.25 +0.3
723,473 + BPHYT_RS23015 0.21 -1.9
723,473 + BPHYT_RS23015 0.21 -0.5
723,902 + BPHYT_RS23015 0.57 -0.6
723,903 - BPHYT_RS23015 0.57 -0.8
724,733 + -1.6
724,733 + -1.8

Or see this region's nucleotide sequence