Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22965 and BPHYT_RS22970 are separated by 82 nucleotides BPHYT_RS22970 and BPHYT_RS22975 are separated by 273 nucleotides BPHYT_RS22975 and BPHYT_RS22980 are separated by 52 nucleotides
BPHYT_RS22965: BPHYT_RS22965 - ligand-gated channel protein, at 710,220 to 712,634
_RS22965
BPHYT_RS22970: BPHYT_RS22970 - hypothetical protein, at 712,717 to 713,127
_RS22970
BPHYT_RS22975: BPHYT_RS22975 - hypothetical protein, at 713,401 to 715,545
_RS22975
BPHYT_RS22980: BPHYT_RS22980 - LysR family transcriptional regulator, at 715,598 to 716,494
_RS22980
Position (kb) 713
714
715
716 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5 at 712.538 kb on - strand at 713.139 kb on + strand at 713.140 kb on - strand at 713.157 kb on + strand at 713.195 kb on + strand at 713.272 kb on + strand at 713.272 kb on + strand at 713.273 kb on - strand at 713.713 kb on + strand, within BPHYT_RS22975 at 713.815 kb on + strand, within BPHYT_RS22975 at 713.846 kb on - strand, within BPHYT_RS22975 at 713.860 kb on + strand, within BPHYT_RS22975 at 714.268 kb on + strand, within BPHYT_RS22975 at 714.269 kb on - strand, within BPHYT_RS22975 at 714.472 kb on + strand, within BPHYT_RS22975 at 714.472 kb on + strand, within BPHYT_RS22975 at 714.479 kb on + strand, within BPHYT_RS22975 at 714.549 kb on - strand, within BPHYT_RS22975 at 715.137 kb on - strand, within BPHYT_RS22975 at 715.324 kb on - strand, within BPHYT_RS22975 at 715.534 kb on - strand at 715.539 kb on + strand at 715.674 kb on + strand at 716.529 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 712,538 - -0.0 713,139 + -0.8 713,140 - -0.6 713,157 + +0.0 713,195 + -0.8 713,272 + -1.6 713,272 + -1.7 713,273 - -2.3 713,713 + BPHYT_RS22975 0.15 +0.8 713,815 + BPHYT_RS22975 0.19 -3.3 713,846 - BPHYT_RS22975 0.21 -1.5 713,860 + BPHYT_RS22975 0.21 -1.2 714,268 + BPHYT_RS22975 0.40 -1.2 714,269 - BPHYT_RS22975 0.40 -1.2 714,472 + BPHYT_RS22975 0.50 +4.8 714,472 + BPHYT_RS22975 0.50 -1.6 714,479 + BPHYT_RS22975 0.50 -0.2 714,549 - BPHYT_RS22975 0.54 -3.5 715,137 - BPHYT_RS22975 0.81 -0.5 715,324 - BPHYT_RS22975 0.90 +0.5 715,534 - -0.4 715,539 + -0.9 715,674 + -0.2 716,529 + +2.8
Or see this region's nucleotide sequence