Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS22965 and BPHYT_RS22970 are separated by 82 nucleotides BPHYT_RS22970 and BPHYT_RS22975 are separated by 273 nucleotides
BPHYT_RS22965: BPHYT_RS22965 - ligand-gated channel protein, at 710,220 to 712,634
_RS22965
BPHYT_RS22970: BPHYT_RS22970 - hypothetical protein, at 712,717 to 713,127
_RS22970
BPHYT_RS22975: BPHYT_RS22975 - hypothetical protein, at 713,401 to 715,545
_RS22975
Position (kb) 712
713
714 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 711.758 kb on - strand, within BPHYT_RS22965 at 711.758 kb on - strand, within BPHYT_RS22965 at 711.763 kb on + strand, within BPHYT_RS22965 at 712.052 kb on - strand, within BPHYT_RS22965 at 712.072 kb on + strand, within BPHYT_RS22965 at 712.135 kb on + strand, within BPHYT_RS22965 at 712.177 kb on + strand, within BPHYT_RS22965 at 712.279 kb on + strand, within BPHYT_RS22965 at 712.279 kb on + strand, within BPHYT_RS22965 at 712.279 kb on + strand, within BPHYT_RS22965 at 712.280 kb on - strand, within BPHYT_RS22965 at 712.280 kb on - strand, within BPHYT_RS22965 at 712.538 kb on - strand at 713.139 kb on + strand at 713.140 kb on - strand at 713.157 kb on + strand at 713.195 kb on + strand at 713.272 kb on + strand at 713.272 kb on + strand at 713.273 kb on - strand at 713.713 kb on + strand, within BPHYT_RS22975 at 713.815 kb on + strand, within BPHYT_RS22975 at 713.846 kb on - strand, within BPHYT_RS22975 at 713.860 kb on + strand, within BPHYT_RS22975
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 711,758 - BPHYT_RS22965 0.64 -0.2 711,758 - BPHYT_RS22965 0.64 +0.4 711,763 + BPHYT_RS22965 0.64 -0.1 712,052 - BPHYT_RS22965 0.76 +0.1 712,072 + BPHYT_RS22965 0.77 -1.0 712,135 + BPHYT_RS22965 0.79 -0.0 712,177 + BPHYT_RS22965 0.81 +0.4 712,279 + BPHYT_RS22965 0.85 -0.3 712,279 + BPHYT_RS22965 0.85 -2.0 712,279 + BPHYT_RS22965 0.85 +3.6 712,280 - BPHYT_RS22965 0.85 -1.6 712,280 - BPHYT_RS22965 0.85 -0.0 712,538 - -0.0 713,139 + -0.8 713,140 - -0.6 713,157 + +0.0 713,195 + -0.8 713,272 + -1.6 713,272 + -1.7 713,273 - -2.3 713,713 + BPHYT_RS22975 0.15 +0.8 713,815 + BPHYT_RS22975 0.19 -3.3 713,846 - BPHYT_RS22975 0.21 -1.5 713,860 + BPHYT_RS22975 0.21 -1.2
Or see this region's nucleotide sequence