Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS22295

Experiment: 36hr PHYTO2 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS22285 and BPHYT_RS22290 are separated by 93 nucleotidesBPHYT_RS22290 and BPHYT_RS22295 are separated by 121 nucleotidesBPHYT_RS22295 and BPHYT_RS22300 overlap by 25 nucleotidesBPHYT_RS22300 and BPHYT_RS22305 are separated by 296 nucleotides BPHYT_RS22285: BPHYT_RS22285 - hypothetical protein, at 556,479 to 556,799 _RS22285 BPHYT_RS22290: BPHYT_RS22290 - membrane protein, at 556,893 to 557,645 _RS22290 BPHYT_RS22295: BPHYT_RS22295 - LysR family transcriptional regulator, at 557,767 to 558,645 _RS22295 BPHYT_RS22300: BPHYT_RS22300 - hypothetical protein, at 558,621 to 559,082 _RS22300 BPHYT_RS22305: BPHYT_RS22305 - hypothetical protein, at 559,379 to 559,573 _RS22305 Position (kb) 557 558 559Strain fitness (log2 ratio) -3 -2 -1 0 1at 556.985 kb on - strand, within BPHYT_RS22290at 556.985 kb on - strand, within BPHYT_RS22290at 557.121 kb on + strand, within BPHYT_RS22290at 557.801 kb on + strandat 558.013 kb on - strand, within BPHYT_RS22295at 558.757 kb on + strand, within BPHYT_RS22300at 558.813 kb on - strand, within BPHYT_RS22300at 559.167 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1
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556,985 - BPHYT_RS22290 0.12 -0.3
556,985 - BPHYT_RS22290 0.12 -0.4
557,121 + BPHYT_RS22290 0.30 -2.8
557,801 + +0.2
558,013 - BPHYT_RS22295 0.28 -0.5
558,757 + BPHYT_RS22300 0.29 -1.0
558,813 - BPHYT_RS22300 0.42 +0.2
559,167 - -1.4

Or see this region's nucleotide sequence