Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21415 and BPHYT_RS21420 are separated by 13 nucleotides BPHYT_RS21420 and BPHYT_RS21425 are separated by 92 nucleotides BPHYT_RS21425 and BPHYT_RS21430 are separated by 5 nucleotides BPHYT_RS21430 and BPHYT_RS21435 overlap by 4 nucleotides
BPHYT_RS21415: BPHYT_RS21415 - 3-oxoadipate CoA-transferase subunit A, at 363,604 to 364,311
_RS21415
BPHYT_RS21420: BPHYT_RS21420 - 3-oxoadipate CoA-transferase subunit B, at 364,325 to 364,984
_RS21420
BPHYT_RS21425: BPHYT_RS21425 - 3-carboxy-cis,cis-muconate cycloisomerase, at 365,077 to 366,456
_RS21425
BPHYT_RS21430: BPHYT_RS21430 - 3-oxoadipate enol-lactonase, at 366,462 to 367,253
_RS21430
BPHYT_RS21435: BPHYT_RS21435 - 4-carboxymuconolactone decarboxylase, at 367,250 to 367,645
_RS21435
Position (kb) 365
366
367 Strain fitness (log2 ratio) -2
-1
0
1 at 364.112 kb on - strand, within BPHYT_RS21415 at 364.388 kb on + strand at 364.414 kb on + strand, within BPHYT_RS21420 at 364.571 kb on + strand, within BPHYT_RS21420 at 364.748 kb on + strand, within BPHYT_RS21420 at 364.844 kb on + strand, within BPHYT_RS21420 at 364.845 kb on - strand, within BPHYT_RS21420 at 365.038 kb on + strand at 365.038 kb on + strand at 365.381 kb on - strand, within BPHYT_RS21425 at 366.373 kb on + strand at 366.373 kb on + strand at 366.374 kb on - strand at 366.374 kb on - strand at 366.394 kb on + strand at 366.394 kb on + strand at 366.469 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 364,112 - BPHYT_RS21415 0.72 +1.3 364,388 + -1.2 364,414 + BPHYT_RS21420 0.13 +1.5 364,571 + BPHYT_RS21420 0.37 +0.8 364,748 + BPHYT_RS21420 0.64 -1.6 364,844 + BPHYT_RS21420 0.79 +0.6 364,845 - BPHYT_RS21420 0.79 -0.2 365,038 + -0.6 365,038 + -1.1 365,381 - BPHYT_RS21425 0.22 -1.4 366,373 + -2.1 366,373 + +0.3 366,374 - -0.4 366,374 - -0.2 366,394 + +0.4 366,394 + -0.2 366,469 - +0.3
Or see this region's nucleotide sequence