Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS21080 and BPHYT_RS21085 overlap by 4 nucleotides BPHYT_RS21085 and BPHYT_RS21090 are separated by 19 nucleotides BPHYT_RS21090 and BPHYT_RS21095 are separated by 335 nucleotides
BPHYT_RS21080: BPHYT_RS21080 - (2Fe-2S)-binding protein, at 290,385 to 291,500
_RS21080
BPHYT_RS21085: BPHYT_RS21085 - hypothetical protein, at 291,497 to 291,706
_RS21085
BPHYT_RS21090: BPHYT_RS21090 - FAD-dependent pyridine nucleotide-disulfide oxidoreductase, at 291,726 to 292,955
_RS21090
BPHYT_RS21095: BPHYT_RS21095 - omega amino acid--pyruvate aminotransferase, at 293,291 to 294,607
_RS21095
Position (kb) 291
292
293 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 290.764 kb on + strand, within BPHYT_RS21080 at 290.862 kb on + strand, within BPHYT_RS21080 at 290.923 kb on - strand, within BPHYT_RS21080 at 291.315 kb on - strand, within BPHYT_RS21080 at 291.867 kb on + strand, within BPHYT_RS21090 at 291.930 kb on + strand, within BPHYT_RS21090 at 291.932 kb on + strand, within BPHYT_RS21090 at 291.972 kb on + strand, within BPHYT_RS21090 at 291.973 kb on - strand, within BPHYT_RS21090 at 292.058 kb on + strand, within BPHYT_RS21090 at 292.059 kb on - strand, within BPHYT_RS21090 at 293.396 kb on + strand at 293.397 kb on - strand at 293.651 kb on - strand, within BPHYT_RS21095 at 293.651 kb on - strand, within BPHYT_RS21095
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 290,764 + BPHYT_RS21080 0.34 -0.5 290,862 + BPHYT_RS21080 0.43 -0.1 290,923 - BPHYT_RS21080 0.48 +2.8 291,315 - BPHYT_RS21080 0.83 -0.4 291,867 + BPHYT_RS21090 0.11 +0.1 291,930 + BPHYT_RS21090 0.17 +0.2 291,932 + BPHYT_RS21090 0.17 -2.3 291,972 + BPHYT_RS21090 0.20 -0.4 291,973 - BPHYT_RS21090 0.20 -0.7 292,058 + BPHYT_RS21090 0.27 +0.4 292,059 - BPHYT_RS21090 0.27 -1.1 293,396 + +0.2 293,397 - +1.0 293,651 - BPHYT_RS21095 0.27 -1.0 293,651 - BPHYT_RS21095 0.27 -1.1
Or see this region's nucleotide sequence