Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20640 and BPHYT_RS20645 overlap by 4 nucleotides BPHYT_RS20645 and BPHYT_RS20650 are separated by 26 nucleotides BPHYT_RS20650 and BPHYT_RS20655 are separated by 73 nucleotides
BPHYT_RS20640: BPHYT_RS20640 - multidrug efflux RND transporter permease subunit, at 189,796 to 192,963
_RS20640
BPHYT_RS20645: BPHYT_RS20645 - RND transporter, at 192,960 to 194,378
_RS20645
BPHYT_RS20650: BPHYT_RS20650 - hypothetical protein, at 194,405 to 194,788
_RS20650
BPHYT_RS20655: BPHYT_RS20655 - hypothetical protein, at 194,862 to 195,305
_RS20655
Position (kb) 192
193
194
195 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 192.058 kb on + strand, within BPHYT_RS20640 at 192.059 kb on - strand, within BPHYT_RS20640 at 192.154 kb on + strand, within BPHYT_RS20640 at 192.352 kb on + strand, within BPHYT_RS20640 at 192.354 kb on + strand, within BPHYT_RS20640 at 192.355 kb on - strand, within BPHYT_RS20640 at 192.358 kb on + strand, within BPHYT_RS20640 at 192.358 kb on + strand, within BPHYT_RS20640 at 193.419 kb on + strand, within BPHYT_RS20645 at 193.485 kb on + strand, within BPHYT_RS20645 at 193.876 kb on - strand, within BPHYT_RS20645 at 194.176 kb on - strand, within BPHYT_RS20645 at 194.462 kb on + strand, within BPHYT_RS20650 at 194.686 kb on + strand, within BPHYT_RS20650 at 194.761 kb on + strand at 194.834 kb on + strand at 194.834 kb on + strand at 194.983 kb on + strand, within BPHYT_RS20655 at 194.984 kb on - strand, within BPHYT_RS20655 at 194.984 kb on - strand, within BPHYT_RS20655 at 194.986 kb on - strand, within BPHYT_RS20655 at 194.986 kb on - strand, within BPHYT_RS20655 at 195.154 kb on + strand, within BPHYT_RS20655
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 192,058 + BPHYT_RS20640 0.71 -0.4 192,059 - BPHYT_RS20640 0.71 -1.2 192,154 + BPHYT_RS20640 0.74 -0.5 192,352 + BPHYT_RS20640 0.81 -0.0 192,354 + BPHYT_RS20640 0.81 -1.4 192,355 - BPHYT_RS20640 0.81 -1.7 192,358 + BPHYT_RS20640 0.81 -1.9 192,358 + BPHYT_RS20640 0.81 +0.7 193,419 + BPHYT_RS20645 0.32 -0.3 193,485 + BPHYT_RS20645 0.37 -0.8 193,876 - BPHYT_RS20645 0.65 -2.8 194,176 - BPHYT_RS20645 0.86 +0.4 194,462 + BPHYT_RS20650 0.15 +0.2 194,686 + BPHYT_RS20650 0.73 +0.1 194,761 + -0.2 194,834 + -0.6 194,834 + +1.4 194,983 + BPHYT_RS20655 0.27 -0.6 194,984 - BPHYT_RS20655 0.27 -1.5 194,984 - BPHYT_RS20655 0.27 -0.2 194,986 - BPHYT_RS20655 0.28 +0.3 194,986 - BPHYT_RS20655 0.28 -0.0 195,154 + BPHYT_RS20655 0.66 +0.2
Or see this region's nucleotide sequence