Experiment: 36hr PHYTO2 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BPHYT_RS20635 and BPHYT_RS20640 are separated by 3 nucleotides BPHYT_RS20640 and BPHYT_RS20645 overlap by 4 nucleotides
BPHYT_RS20635: BPHYT_RS20635 - RND transporter, at 188,110 to 189,792
_RS20635
BPHYT_RS20640: BPHYT_RS20640 - multidrug efflux RND transporter permease subunit, at 189,796 to 192,963
_RS20640
BPHYT_RS20645: BPHYT_RS20645 - RND transporter, at 192,960 to 194,378
_RS20645
Position (kb) 189
190
191
192
193 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 188.863 kb on + strand, within BPHYT_RS20635 at 188.924 kb on - strand, within BPHYT_RS20635 at 189.064 kb on + strand, within BPHYT_RS20635 at 189.318 kb on + strand, within BPHYT_RS20635 at 189.318 kb on + strand, within BPHYT_RS20635 at 189.319 kb on - strand, within BPHYT_RS20635 at 189.946 kb on + strand at 190.030 kb on + strand at 190.031 kb on - strand at 190.031 kb on - strand at 190.031 kb on - strand at 190.063 kb on - strand at 190.079 kb on - strand at 190.283 kb on - strand, within BPHYT_RS20640 at 190.299 kb on - strand, within BPHYT_RS20640 at 191.207 kb on - strand, within BPHYT_RS20640 at 191.650 kb on + strand, within BPHYT_RS20640 at 191.651 kb on - strand, within BPHYT_RS20640 at 191.950 kb on - strand, within BPHYT_RS20640 at 192.058 kb on + strand, within BPHYT_RS20640 at 192.059 kb on - strand, within BPHYT_RS20640 at 192.154 kb on + strand, within BPHYT_RS20640 at 192.352 kb on + strand, within BPHYT_RS20640 at 192.354 kb on + strand, within BPHYT_RS20640 at 192.355 kb on - strand, within BPHYT_RS20640 at 192.358 kb on + strand, within BPHYT_RS20640 at 192.358 kb on + strand, within BPHYT_RS20640 at 193.419 kb on + strand, within BPHYT_RS20645 at 193.485 kb on + strand, within BPHYT_RS20645 at 193.876 kb on - strand, within BPHYT_RS20645
Per-strain Table
Position Strand Gene LocusTag Fraction 36hr PHYTO2 MOI 1 remove 188,863 + BPHYT_RS20635 0.45 -1.4 188,924 - BPHYT_RS20635 0.48 -2.0 189,064 + BPHYT_RS20635 0.57 +0.1 189,318 + BPHYT_RS20635 0.72 -0.9 189,318 + BPHYT_RS20635 0.72 -0.3 189,319 - BPHYT_RS20635 0.72 -0.7 189,946 + +0.6 190,030 + +0.3 190,031 - -0.2 190,031 - -0.1 190,031 - +1.6 190,063 - -0.4 190,079 - -0.5 190,283 - BPHYT_RS20640 0.15 -0.7 190,299 - BPHYT_RS20640 0.16 +0.0 191,207 - BPHYT_RS20640 0.45 +0.1 191,650 + BPHYT_RS20640 0.59 -1.0 191,651 - BPHYT_RS20640 0.59 +0.6 191,950 - BPHYT_RS20640 0.68 -0.8 192,058 + BPHYT_RS20640 0.71 -0.4 192,059 - BPHYT_RS20640 0.71 -1.2 192,154 + BPHYT_RS20640 0.74 -0.5 192,352 + BPHYT_RS20640 0.81 -0.0 192,354 + BPHYT_RS20640 0.81 -1.4 192,355 - BPHYT_RS20640 0.81 -1.7 192,358 + BPHYT_RS20640 0.81 -1.9 192,358 + BPHYT_RS20640 0.81 +0.7 193,419 + BPHYT_RS20645 0.32 -0.3 193,485 + BPHYT_RS20645 0.37 -0.8 193,876 - BPHYT_RS20645 0.65 -2.8
Or see this region's nucleotide sequence